{"doi":"10.1101/2025.01.13.632696","title":"ATG-3 limits Orsay virus infection in <i>C. elegans</i> through regulation of collagen pathways","abstract":"ABSTRACT Autophagy is an essential cellular process which functions to maintain homeostasis in response to stressors such as starvation or infection. Here, we report that a subset of autophagy factors including ATG-3 play an antiviral role in Orsay virus infection of Caenorhabditis elegans . Orsay virus infection does not modulate autophagic flux, and re-feeding after starvation limits Orsay virus infection and blocks autophagic flux, suggesting that the role of ATG-3 in Orsay virus susceptibility is independent of its role in maintaining autophagic flux. atg-3 mutants phenocopy rde-1 mutants, which have a defect in RNA interference (RNAi), in susceptibility to Orsay virus infection and transcriptional response to infection. However, atg-3 mutants do not exhibit defects in RNAi. Additionally, atg-3 limits viral infection at a post-entry step, similar to rde-1 mutants. Differential expression analysis using RNA sequencing revealed that antiviral sqt-2 , which encodes a collagen trimer protein, is depleted in naïve and infected atg-3 mutants, as well as in infected WT animals, as are numerous other collagen genes. These data suggest that ATG-3 has a role in collagen organization pathways that function in antiviral defense in C. elegans .","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":557073,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9572,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1264426,"name":"Vivien Marmerstein","orcid":null,"position":1,"is_corresponding":false},{"id":716507,"name":"Lawrence A. Schriefer","orcid":"0000-0001-6865-8683","position":2,"is_corresponding":false},{"id":413851,"name":"Leran Wang","orcid":"0009-0003-6928-9893","position":3,"is_corresponding":false},{"id":511645,"name":"Stephanie Morrison","orcid":"0000-0001-8321-6744","position":4,"is_corresponding":false},{"id":1457182,"name":"Luis Casorla Perez","orcid":null,"position":5,"is_corresponding":false},{"id":246656,"name":"Tim Schedl","orcid":"0000-0003-2148-2996","position":6,"is_corresponding":false},{"id":485359,"name":"Stephen C. Pak","orcid":"0000-0002-8089-3237","position":7,"is_corresponding":false},{"id":298001,"name":"Megan T. Baldridge","orcid":"0000-0002-7030-6131","position":8,"is_corresponding":false},{"id":618070,"name":"Gowri Kalugotla","orcid":"0000-0002-0777-8275","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-19T02:55:13.130091Z","pmid":"39868230","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}