{"doi":"10.1101/2025.01.07.631699","title":"SDS-22 stabilizes the PP1 catalytic subunits GSP-1/-2 contributing to polarity establishment in <i>C. elegans</i> embryos","abstract":"Abstract In many cells, cell polarity depends on the asymmetric distribution of the conserved PAR proteins, maintained by a balanced activity between kinases and phosphatases. The C. elegans one-cell embryo is polarized along the anterior-posterior axis, with the atypical protein kinase C PKC-3 enriched in the anterior, and the ring finger protein PAR-2 in the posterior. PAR-2 localization is regulated by PKC-3 and the PP1 phosphatases GSP-1/-2. Here, we find that, similar to GSP-2 depletion, depletion of the conserved PP1 interactor SDS-22 results in a partial rescue of the polarity defects of a pkc-3 temperature-sensitive mutant. Consistent with the rescue, SDS-22 depletion or mutation results in reduced GSP - 1/ - 2 protein levels and activity. The decreased levels of GSP-1/-2 can be rescued by reducing proteasomal activity. Our data suggest that SDS-22 contributes to polarity by protecting the GSP-1 and GSP-2 catalytic subunits from proteasome-mediated degradation, supporting recent data in human cells showing the SDS22 is required to stabilize nascent PP1.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":560132,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9541,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":954287,"name":"Ida Calvi","orcid":"0000-0002-4416-5574","position":1,"is_corresponding":false},{"id":716743,"name":"Monica Gotta","orcid":"0000-0002-4336-7860","position":2,"is_corresponding":false},{"id":1462281,"name":"Yi Li","orcid":"0009-0008-3904-8892","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-19T02:55:42.883572Z","pmid":"40654901","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}