{"doi":"10.1101/2024.12.20.629462","title":"Genomic Specificity of Anti-TCR mAbs determined by single-cell RNAseq","abstract":"ABSTRACT T cells play a pivotal role in the immune system, relying on their somatically rearranged T cell receptor (TCR) to recognize peptide-MHC complexes. A comprehensive and extensively used set of monoclonal antibodies (mAbs) against TCR Variable regions was generated in the previous century. The separate identification of mAb-specific TCR-V proteins and TRV genes has resulted in multiple nomenclatures, making their relationships unclear. To formally re-establish this link and determine patterns of reactivity within TRV subfamilies, we sorted T cells positive for any one of a panel of 22 anti-V mAbs and determined their TRV genes by single-cell TCRseq. RNAseq data revealed consistently higher expression of repeated elements from the ERV1-family LTR RLTR6Mm (mapping to Gm20400 ) in cells utilizing TRBV segments encoded within a 66kb genomic region between TRBV23 and TRBV30. O ur findings provide a comprehensive resource for anti-TCR mAb specificity and insight into V-gene usage biases and T cell function.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":507991,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9458,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1360079,"name":"Val Piekarsa","orcid":null,"position":1,"is_corresponding":false},{"id":1245073,"name":"Sofia Kossida","orcid":"0000-0003-2482-0022","position":2,"is_corresponding":false},{"id":243364,"name":"Joshua Croteau","orcid":"0000-0002-8142-0482","position":3,"is_corresponding":false},{"id":266203,"name":"Amy Huei‐Yi Lee","orcid":"0000-0003-3250-3246","position":4,"is_corresponding":false},{"id":65553,"name":"Robert Balderas","orcid":"0000-0002-8734-8629","position":5,"is_corresponding":false},{"id":21637,"name":"David Zemmour","orcid":"0000-0002-0990-217X","position":6,"is_corresponding":false},{"id":18715,"name":"Christophe Benoıst","orcid":"0000-0003-1172-6555","position":7,"is_corresponding":false},{"id":1360080,"name":"the immgenT Project","orcid":null,"position":8,"is_corresponding":false},{"id":1169900,"name":"Ian Magill","orcid":"0009-0009-1280-3365","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-19T02:11:06.395600Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}