{"doi":"10.1101/2024.12.16.628673","title":"PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities","abstract":"Abstract Genetically encoded calcium (Ca 2+ ) indicators (GECIs) are widely used for imaging neuronal activity, yet current limitations of existing red fluorescent GECIs have constrained their applicability. The inherently dim fluorescence and low signal-to-noise ratio of red-shifted GECIs have posed significant challenges. More critically, several red-fluorescent GECIs exhibit photoswitching when exposed to blue light, thereby limiting their applicability in all-optical experimental approaches. Here, we present the development of PinkyCaMP, the first mScarlet-based Ca 2+ sensor that outperforms current red fluorescent sensors in brightness, photostability, signal-to-noise ratio, and compatibility with optogenetics and neurotransmitter imaging. PinkyCaMP is well-tolerated by neurons, showing no toxicity or aggregation, both in vitro and in vivo . All imaging approaches, including single-photon excitation methods such as fiber photometry, widefield imaging, miniscope imaging, as well as two-photon imaging in awake mice, are fully compatible with PinkyCaMP.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":484487,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9504,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1226006,"name":"Shosei Imai","orcid":"0000-0003-0388-4764","position":1,"is_corresponding":false},{"id":1326656,"name":"Nala Gockel","orcid":"0009-0004-9464-0224","position":2,"is_corresponding":false},{"id":1326657,"name":"German Lauer","orcid":"0000-0002-4588-2743","position":3,"is_corresponding":false},{"id":1326658,"name":"Kim Renken","orcid":"0009-0004-4644-7673","position":4,"is_corresponding":false},{"id":1169326,"name":"Jonas Wietek","orcid":"0000-0002-8079-7042","position":5,"is_corresponding":false},{"id":1160384,"name":"Paul J. 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