{"doi":"10.1101/2024.12.06.625234","title":"Defining a highly conserved B cell epitope in the receptor binding motif of SARS-CoV-2 spike glycoprotein","abstract":"SARS-CoV-2 mRNA vaccines induce robust and persistent germinal centre (GC) B cell responses in humans. It remains unclear how the continuous evolution of the virus impacts the breadth of the induced GC B cell response. Using ultrasound-guided fine needle aspiration, we examined draining lymph nodes of nine healthy adults following bivalent booster immunization. We show that 77.8% of the B cell clones in the GC expressed as representative monoclonal antibodies recognized the spike protein, with a third (37.8%) of these targeting the receptor binding domain (RBD). Strikingly, only one RBD-targeting mAb, mAb-52, neutralized all tested SARS-CoV-2 strains, including the recent KP.2 variant. mAb-52 utilizes the IGHV3-66 public clonotype, protects hamsters challenged against the EG.5.1 variant and targets the class I/II RBD epitope, closely mimicking the binding footprint of ACE2. Finally, we show that the remarkable breadth of mAb-52 is due to the somatic hypermutations accumulated within vaccine-induced GC reaction.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":489373,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9572,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1334267,"name":"Deepika Jaiswal","orcid":"0000-0002-9565-1302","position":1,"is_corresponding":false},{"id":408059,"name":"Baoling Ying","orcid":null,"position":2,"is_corresponding":false},{"id":225531,"name":"Wafaa B. Alsoussi","orcid":"0000-0002-5805-4461","position":3,"is_corresponding":false},{"id":252252,"name":"Astha Joshi","orcid":"0000-0003-4914-8228","position":4,"is_corresponding":false},{"id":839941,"name":"Bernadeta Dadonaite","orcid":"0000-0003-0908-6982","position":5,"is_corresponding":false},{"id":1334705,"name":"Alesandro Civljak","orcid":null,"position":6,"is_corresponding":false},{"id":805454,"name":"Stephen Horváth","orcid":"0000-0001-5324-1666","position":7,"is_corresponding":false},{"id":242710,"name":"Julian Q. Zhou","orcid":"0000-0001-9602-2092","position":8,"is_corresponding":false},{"id":614787,"name":"Wooseob Kim","orcid":"0000-0002-9199-1000","position":9,"is_corresponding":false},{"id":225532,"name":"Jackson S. Turner","orcid":"0000-0002-4396-6265","position":10,"is_corresponding":false},{"id":225533,"name":"Aaron J. Schmitz","orcid":"0000-0002-8077-6751","position":11,"is_corresponding":false},{"id":983807,"name":"Fangjie Han","orcid":null,"position":12,"is_corresponding":false},{"id":689736,"name":"Suzanne M. Scheaffer","orcid":"0009-0001-0459-0816","position":13,"is_corresponding":false},{"id":555572,"name":"Christopher W Farnsworth","orcid":"0000-0001-7169-3850","position":14,"is_corresponding":false},{"id":123992,"name":"Raffael Nachbagauer","orcid":"0000-0001-5568-5420","position":15,"is_corresponding":false},{"id":983809,"name":"Biliana Nestorova","orcid":null,"position":16,"is_corresponding":false},{"id":838767,"name":"Spyros Chalkias","orcid":"0000-0002-6254-1158","position":17,"is_corresponding":false},{"id":12097,"name":"Michael K. Klebert","orcid":"0000-0003-3329-3674","position":18,"is_corresponding":false},{"id":105569,"name":"Darin K. Edwards","orcid":"0000-0002-2065-2941","position":19,"is_corresponding":false},{"id":593255,"name":"Robert Paris","orcid":"0000-0003-3234-5989","position":20,"is_corresponding":false},{"id":983243,"name":"Benjamin S. Strnad","orcid":"0000-0003-2308-1299","position":21,"is_corresponding":false},{"id":285661,"name":"William D. Middleton","orcid":"0000-0002-9869-1107","position":22,"is_corresponding":false},{"id":259653,"name":"Jane A. O’Halloran","orcid":"0000-0001-8265-9471","position":23,"is_corresponding":false},{"id":552303,"name":"Rachel M. Presti","orcid":"0000-0002-5469-0727","position":24,"is_corresponding":false},{"id":106715,"name":"Jesse D. Bloom","orcid":"0000-0003-1267-3408","position":25,"is_corresponding":false},{"id":334926,"name":"Adrianus C. M. Boon","orcid":"0000-0002-4700-8224","position":26,"is_corresponding":false},{"id":106529,"name":"Michael Diamond","orcid":"0000-0002-8791-3165","position":27,"is_corresponding":false},{"id":490757,"name":"Goran Bajic","orcid":"0000-0003-0480-4324","position":28,"is_corresponding":false},{"id":225537,"name":"Ali H. Ellebedy","orcid":"0000-0002-6129-2532","position":29,"is_corresponding":false},{"id":439801,"name":"Sameer Kumar Malladi","orcid":"0000-0001-5131-4518","position":0,"is_corresponding":true}],"reference_count":86,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:08:23.929823Z","pmid":"39713327","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}