{"doi":"10.1101/2024.11.04.621940","title":"Characterization of A Bronchoscopically Induced Transgenic Lung Cancer Pig Model for Human Translatability","abstract":"ABSTRACT Background There remains a need for animal models with human translatability in lung cancer (LC) research. Findings in pigs have high impact on humans due to similar anatomy and physiology. We present the characterization of a bronchoscopically-induced LC model in Oncopigs carrying inducible KRAS G12D and TP53 R167H mutations. Methods Twelve Oncopigs underwent 29 injections via flexible bronchoscopy. Eighteen Adenovirus-Cre recombinase gene (AdCre) inductions were performed endobronchially (n=6) and transbronchially with a needle (n=12). Eleven control injections were performed without AdCre. Oncopigs underwent serial contrast-enhanced chest CT with clinical follow-up for 29 weeks. Following autopsy, lung and organ tissues underwent histopathology, immunohistochemistry, and RNA-sequencing with comparative analysis with The Cancer Genome Atlas (TCGA) human LC data. Results All 18 sites of AdCre injections had lung consolidations on CT imaging. Transbronchial injections led to histopathologic invasive cancer and/or carcinoma in situ (CIS) in 11/12 (91.7%), and invasive cancer (excluding CIS) in 8/12 (66.6%). Endobronchial inductions led to invasive cancer in 3/6 (50%). A soft tissue metastasis was observed in one Oncopig. Immunohistochemistry confirmed expression of Pan-CK+/epithelial cancer cells, with macrophages and T cells infiltration in the tumor microenvironment. Transcriptome comparison showed 54.3% overlap with human LC (TCGA), in contrast to 29.88% overlap of KRAS-mutant mouse LC with human LC. Conclusions The transgenic and immunocompetent Oncopig model has a high rate of LC following bronchoscopic transbronchial induction. Overlap of the Oncopig LC transcriptome with human LC transcriptome was noted. This pig model is expected to have high clinical translatability to the human LC patient.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":505860,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9068,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":772356,"name":"Kanve N. Suvilesh","orcid":"0000-0002-8054-7027","position":1,"is_corresponding":false},{"id":778241,"name":"Nagabhishek Sirpu Natesh","orcid":"0000-0003-3826-1591","position":2,"is_corresponding":false},{"id":772355,"name":"Yariswamy Manjunath","orcid":"0000-0003-1833-6998","position":3,"is_corresponding":false},{"id":1357219,"name":"Jared Coberly","orcid":null,"position":4,"is_corresponding":false},{"id":1269019,"name":"Sarah Schlink","orcid":null,"position":5,"is_corresponding":false},{"id":1357220,"name":"Jeffrey Kunin","orcid":null,"position":6,"is_corresponding":false},{"id":393506,"name":"Randall S. Prather","orcid":"0000-0002-6012-4035","position":7,"is_corresponding":false},{"id":465064,"name":"Kristin M. Whitworth","orcid":"0000-0001-9959-108X","position":8,"is_corresponding":false},{"id":1237358,"name":"Benjamin Nelson","orcid":null,"position":9,"is_corresponding":false},{"id":660238,"name":"Jeffrey N. Bryan","orcid":"0000-0002-6820-9850","position":10,"is_corresponding":false},{"id":1218343,"name":"Timothy J. Hoffman","orcid":"0000-0002-8580-3034","position":11,"is_corresponding":false},{"id":921865,"name":"Mojgan Golzy","orcid":"0000-0002-2759-3120","position":12,"is_corresponding":false},{"id":1332714,"name":"Murugesan Raju","orcid":"0000-0002-2618-8027","position":13,"is_corresponding":false},{"id":298763,"name":"Emma Teixeiro","orcid":"0000-0002-3596-5481","position":14,"is_corresponding":false},{"id":364486,"name":"Bhanu P. Telugu","orcid":"0000-0002-7288-7750","position":15,"is_corresponding":false},{"id":772357,"name":"Jussuf T. Kaifi","orcid":"0000-0003-3425-2356","position":16,"is_corresponding":false},{"id":255892,"name":"Satyanarayana Rachagani","orcid":"0000-0003-3949-8566","position":18,"is_corresponding":false},{"id":449559,"name":"Kirtan Joshi","orcid":"0000-0001-9176-7378","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":null,"created_at":"2026-07-19T02:10:50.984927Z","pmid":"39569144","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}