{"doi":"10.1101/2024.10.30.621210","title":"Metabolic Control of Glycosylation Forms for Establishing Glycan-Dependent Protein Interaction Networks","abstract":"Protein-protein interactions (PPIs) provide essential insights into the complex molecular mechanisms and signaling pathways within cells that regulate development and disease-related phenotypes. However, for membrane proteins, the impact of various forms of glycosylation has often been overlooked in PPI studies. In this study, we introduce a novel approach, glycan-dependent affinity purification followed by mass spectrometry (GAP-MS), to assess variations in PPIs for any glycoprotein of interest under different glycosylation conditions. As a proof of principle, we selected four glycoproteins-BSG, CD44, EGFR, and SLC3A2-as baits to compare their co-purified partners across five metabolically controlled glycan conditions. The findings demonstrate the capability of GAP-MS to identify PPIs influenced by altered glycosylation states, establishing a foundation for systematically exploring the Glycan-Dependent Protein Interactome (GDPI) for other glycoproteins of interest.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":492284,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9628,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1338110,"name":"Li Yi","orcid":"0009-0002-2872-2569","position":1,"is_corresponding":false},{"id":582407,"name":"Zongtao Lin","orcid":"0000-0002-6017-338X","position":2,"is_corresponding":false},{"id":694182,"name":"Siyu Chen","orcid":"0009-0003-9584-6905","position":3,"is_corresponding":false},{"id":562119,"name":"Shunyang Wang","orcid":"0000-0001-9851-2968","position":4,"is_corresponding":false},{"id":456102,"name":"Ying Sheng","orcid":"0000-0002-6002-9783","position":5,"is_corresponding":false},{"id":235510,"name":"Carlito B. Lebrilla","orcid":"0000-0001-7190-5323","position":6,"is_corresponding":false},{"id":74960,"name":"Benjamin A. Garcia","orcid":"0000-0003-3596-4750","position":7,"is_corresponding":false},{"id":407239,"name":"Yixuan Xie","orcid":"0000-0002-8512-6053","position":8,"is_corresponding":false},{"id":1252878,"name":"Xingyu Liu","orcid":"0000-0003-0008-7815","position":0,"is_corresponding":true}],"reference_count":42,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:08:49.768795Z","pmid":"39554187","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}