{"doi":"10.1101/2024.10.15.618486","title":"Human Anti-Glycan Reactivity is Driven by the Selection of B cells Utilizing Private Antibody Gene Rearrangements that are Affinity Maturated in Germinal Centers","abstract":"Abstract The human antibody repertoire is broadly reactive with carbohydrate antigens represented in the universe of all living things, including both the host/self-as well as the commensal microflora-derived glycomes. Here we have used BCR receptor cloning and expression together with single-cell transcriptomics to analyze the B cell repertoire to the ubiquitous N-acetyl-D-glucosamine (GlcNAc) epitope in human cohorts and dissect the immune phylogeny of this predominant class of antibodies. We find that circulating anti-GlcNAc B cells exhibiting canonical BMem phenotypes emerge rapidly after birth and couple this observation with evidence for germinal center-dependent affinity maturation of carbohydrate-specific B cell receptors in situ during early childhood. Direct analysis of individual B cell clonotypes reveals they exhibit strikingly distinct fine-specificity profiles for palettes of GlcNAc containing moieties. These results suggest that a generalized exposure to complex environmental glycans drives the steady state anti-glycan repertoire.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":489168,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9483,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":181722,"name":"Christopher Fucile","orcid":null,"position":1,"is_corresponding":false},{"id":1171762,"name":"A. Callahan","orcid":null,"position":2,"is_corresponding":false},{"id":1334539,"name":"J. C. Burke","orcid":null,"position":3,"is_corresponding":false},{"id":720670,"name":"Randall S. Davis","orcid":"0000-0003-1906-8219","position":4,"is_corresponding":false},{"id":762511,"name":"Wayne Duck","orcid":null,"position":5,"is_corresponding":false},{"id":293771,"name":"Alexander F. Rosenberg","orcid":null,"position":6,"is_corresponding":false},{"id":292531,"name":"John F. Kearney","orcid":"0000-0003-2569-2560","position":7,"is_corresponding":false},{"id":292532,"name":"R. Glenn King","orcid":"0000-0001-6522-0053","position":8,"is_corresponding":false},{"id":293770,"name":"James S. New","orcid":null,"position":0,"is_corresponding":true}],"reference_count":51,"raw_metadata":null,"created_at":"2026-07-19T02:08:23.929823Z","pmid":"39464096","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}