{"doi":"10.1101/2024.10.02.616386","title":"Immunopeptidomics informs discovery and delivery of <i>Mycobacterium tuberculosis</i> MHC-II antigens for vaccine design","abstract":"Abstract No currently licensed vaccine reliably prevents pulmonary tuberculosis (TB), a leading cause of infectious disease mortality. Developing effective new vaccines will require identifying which of the roughly 4000 proteins in the Mycobacterium tuberculosis ( Mtb ) proteome are presented on MHC class II (MHC-II) by infected human phagocytes and can be recognized by CD4+ T cells to mediate protective immunity. Vaccines must also elicit T cell responses recognizing the same peptide-MHC complexes presented by infected cells, and successful presentation of target human MHC-II peptides is currently challenging to evaluate and optimize. Here, we define antigenic targets for TB vaccine development by using mass spectrometry (MS) for proteome-wide discovery of Mtb epitopes presented on MHC-II by infected human cells. We next iteratively design and evaluate candidate mRNA vaccine immunogens, revealing design principles that enhance presentation of target MHC-II peptides. Our results will inform the development of new TB vaccine candidates.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":489102,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9515,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":266674,"name":"Paul Ogongo","orcid":"0000-0002-0093-5768","position":1,"is_corresponding":false},{"id":1217108,"name":"Julia Huffaker","orcid":"0009-0008-8939-1971","position":2,"is_corresponding":false},{"id":1194191,"name":"Mingyu Gan","orcid":"0009-0008-4405-092X","position":3,"is_corresponding":false},{"id":1214853,"name":"Ryan O. Milligan","orcid":"0000-0001-5031-1892","position":4,"is_corresponding":false},{"id":1334007,"name":"Sheikh Mahmud","orcid":"0009-0006-6063-7259","position":5,"is_corresponding":false},{"id":292650,"name":"Yuko Yuki","orcid":"0000-0003-1721-6003","position":6,"is_corresponding":false},{"id":929606,"name":"Kidist Bobosha","orcid":"0000-0001-8091-7182","position":7,"is_corresponding":false},{"id":487247,"name":"Liya Wassie","orcid":"0000-0002-5494-5275","position":8,"is_corresponding":false},{"id":37727,"name":"Mary Carrington","orcid":"0000-0002-2692-2180","position":9,"is_corresponding":false},{"id":459374,"name":"Qingyun Liu","orcid":"0000-0002-2284-5050","position":10,"is_corresponding":false},{"id":8685,"name":"J. Ernst","orcid":"0000-0001-9951-6207","position":11,"is_corresponding":false},{"id":225709,"name":"Forest M. White","orcid":"0000-0002-1545-1651","position":12,"is_corresponding":false},{"id":91622,"name":"Bryan D. Bryson","orcid":"0000-0003-1716-6712","position":13,"is_corresponding":false},{"id":716880,"name":"Owen Leddy","orcid":"0000-0002-3437-1956","position":0,"is_corresponding":true}],"reference_count":90,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:08:23.929823Z","pmid":"41031036","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}