{"doi":"10.1101/2024.09.20.614154","title":"Intranasal immunization with CPAF combined with cyclic-di-AMP induces a memory CD4 T cell response and reduces bacterial burden following intravaginal infection with <i>Chlamydia muridarum</i>","abstract":"ABSTRACT Chlamydia trachomatis (Ct) is the most common bacterial sexually transmitted infection globally, and a vaccine is urgently needed to stop transmission and disease. Chlamydial Protease Activity Factor (CPAF) is an immunoprevalent and immunodominant antigen for CD4 T cells and B cells, which makes it a strong vaccine candidate. Due to the tolerogenic nature of the female genital tract (FGT) and its lack of secondary lymphoid tissue, effective induction of protective cell-mediated immunity will likely require potent and safe mucosal adjuvants. To address this need, we produced CPAF in a cell-free protein synthesis platform and adjuvanted it with the TLR9-agonist CpG1826, STING (stimulator of interferon genes) agonist cyclic-di-AMP (CDA), and/or the squalene oil-in-water nanoemulsion, AddaS03. We determined that intranasal immunization with CPAF plus CDA was well tolerated in female mice, induced CD4 T cells that produced IL-17A or IFNγ, significantly reduced bacterial shedding, and shortened the duration of infection in mice intravaginally challenged with Chlamydia muridarum . These data demonstrate the potential for CDA as a mucosal adjuvant for vaccines against Chlamydia genital tract infection.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":503699,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9612,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1353506,"name":"Jenna Girardi","orcid":"0000-0002-2969-0207","position":1,"is_corresponding":false},{"id":1231139,"name":"Marie Kim","orcid":"0000-0002-3373-6618","position":2,"is_corresponding":false},{"id":1231688,"name":"Peter Zwarycz","orcid":null,"position":3,"is_corresponding":false},{"id":1231689,"name":"A Grace Polson","orcid":null,"position":4,"is_corresponding":false},{"id":926862,"name":"Kacy S. Yount","orcid":"0000-0001-7050-0164","position":5,"is_corresponding":false},{"id":1231690,"name":"Courtne Hanlan","orcid":null,"position":6,"is_corresponding":false},{"id":1231691,"name":"Ian Jaras Salas","orcid":null,"position":7,"is_corresponding":false},{"id":1231692,"name":"Sarah Mae Lammert","orcid":null,"position":8,"is_corresponding":false},{"id":1231693,"name":"Daisy Arroyo","orcid":null,"position":9,"is_corresponding":false},{"id":1231694,"name":"Tony Bruno","orcid":null,"position":10,"is_corresponding":false},{"id":555733,"name":"Manhong Wu","orcid":"0000-0001-5491-3081","position":11,"is_corresponding":false},{"id":637458,"name":"James Rozzelle","orcid":null,"position":12,"is_corresponding":false},{"id":460573,"name":"Jeff Fairman","orcid":"0000-0001-5763-2867","position":13,"is_corresponding":false},{"id":464966,"name":"Aaron P. Esser‐Kahn","orcid":"0000-0003-1273-0951","position":14,"is_corresponding":false},{"id":296481,"name":"Toni Darville","orcid":null,"position":15,"is_corresponding":false},{"id":408590,"name":"Taylor B. Poston","orcid":"0000-0002-5985-4153","position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:10:31.826282Z","pmid":"39386616","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}