{"doi":"10.1101/2024.08.08.607276","title":"The molecular structure of an axle-less F <sub>1</sub> -ATPase","abstract":"Abstract F 1 F o ATP synthase is a molecular rotary motor that can generate ATP using a transmembrane proton motive force. Isolated F 1 -ATPase catalytic cores can hydrolyse ATP, passing through a series of conformational states involving rotation of the central γ rotor subunit and the opening and closing of the catalytic β subunits. Cooperativity in F 1 -ATPase has long thought to be conferred through the γ subunit, with three key interaction sites between the γ and β subunits being identified. Single molecule studies have demonstrated that the F 1 complexes lacking the γ axle still “rotate” and hydrolyse ATP, but with less efficiency. We solved the cryogenic electron microscopy structure of an axle-less Bacillus sp. PS3 F 1 -ATPase. The unexpected binding-dwell conformation of the structure in combination with the observed lack of interactions between the axle-less γ and the open β subunit suggests that the complete γ subunit is important for coordinating efficient ATP binding of F 1 -ATPase.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":501957,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9649,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1273754,"name":"Ian-Blaine P. Reininger-Chatzigiannakis","orcid":null,"position":1,"is_corresponding":false},{"id":267450,"name":"Yi C. Zeng","orcid":"0000-0001-9964-0929","position":2,"is_corresponding":false},{"id":816345,"name":"Simon H. J. Brown","orcid":"0000-0003-3997-4614","position":3,"is_corresponding":false},{"id":267448,"name":"Meghna Sobti","orcid":"0000-0001-7086-3467","position":4,"is_corresponding":false},{"id":267452,"name":"Alastair G. Stewart","orcid":"0000-0002-2070-6030","position":5,"is_corresponding":false},{"id":634060,"name":"Emily Furlong","orcid":"0000-0002-9086-2979","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:10:19.647285Z","pmid":"39149353","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}