{"doi":"10.1101/2024.08.08.607227","title":"A synthetic cell-free pathway for biocatalytic upgrading of one-carbon substrates","abstract":"Abstract Biotechnological processes hold tremendous potential for the efficient and sustainable conversion of one-carbon (C1) substrates into complex multi-carbon products. However, the development of robust and versatile biocatalytic systems for this purpose remains a significant challenge. In this study, we report a hybrid electrochemical-biochemical cell-free system for the conversion of C1 substrates into the universal biological building block acetyl-CoA. The synthetic reductive formate pathway (ReForm) consists of five core enzymes catalyzing non-natural reactions that were established through a cell-free enzyme engineering platform. We demonstrate that ReForm works in a plug-and-play manner to accept diverse C1 substrates including CO 2 equivalents. We anticipate that ReForm will facilitate efforts to build and improve synthetic C1 utilization pathways for a formate-based bioeconomy.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":485662,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9475,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":355764,"name":"Bastian Vögeli","orcid":"0000-0001-7870-7311","position":1,"is_corresponding":false},{"id":1328687,"name":"Cong Tian","orcid":"0000-0002-1260-3402","position":2,"is_corresponding":false},{"id":1218652,"name":"Bharti Singal","orcid":"0000-0002-9482-5251","position":3,"is_corresponding":false},{"id":1044377,"name":"Anika Gupta","orcid":"0000-0001-6349-6742","position":4,"is_corresponding":false},{"id":1329173,"name":"Rebeca Lion","orcid":null,"position":5,"is_corresponding":false},{"id":344088,"name":"Edward H. Sargent","orcid":"0000-0003-0396-6495","position":6,"is_corresponding":false},{"id":268958,"name":"Ashty S. Karim","orcid":"0000-0002-5789-7715","position":7,"is_corresponding":false},{"id":242369,"name":"Michael C. Jewett","orcid":"0000-0003-2948-6211","position":8,"is_corresponding":false},{"id":1328686,"name":"Grant M. Landwehr","orcid":"0000-0003-0953-0318","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-19T02:07:52.536246Z","pmid":"39149402","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}