{"doi":"10.1101/2024.07.19.604361","title":"Lactate-carried Mitochondrial Energy Overflow","abstract":"Abstract We addressed the question of mitochondrial lactate metabolism using genetically-encoded sensors. The organelle was found to contain a dynamic lactate pool that leads to dose- and time-dependent protein lactylation. In neurons, mitochondrial lactate reported blood lactate levels with high fidelity. The exchange of lactate across the inner mitochondrial membrane was found to be mediated by a high affinity H + -coupled transport system involving the mitochondrial pyruvate carrier MPC. Assessment of electron transport chain activity and determination of lactate flux showed that mitochondria are tonic lactate producers, a phenomenon driven by energization and stimulated by hypoxia. We conclude that an overflow mechanism caps the redox level of mitochondria, while saving energy in the form of lactate. One Sentence Summary Mitochondrial lactate production","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":486271,"datarank":0.27752245989693275,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.03610677303181766,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.03610677303181766,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":3,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9545,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1330066,"name":"Yasna Contreras-Baeza","orcid":null,"position":1,"is_corresponding":false},{"id":1330067,"name":"Hugo Faurand","orcid":null,"position":2,"is_corresponding":false},{"id":1330068,"name":"Nataly Cárcamo","orcid":null,"position":3,"is_corresponding":false},{"id":1330069,"name":"Raibel Suárez","orcid":null,"position":4,"is_corresponding":false},{"id":1329671,"name":"Alexandra von Faber-Castell","orcid":"0000-0002-9035-5694","position":5,"is_corresponding":false},{"id":1330070,"name":"F. Ferreira da Silva","orcid":null,"position":6,"is_corresponding":false},{"id":1330071,"name":"Valentina Mora-González","orcid":null,"position":7,"is_corresponding":false},{"id":941904,"name":"Matthias T. Wyss","orcid":"0000-0002-2037-3424","position":8,"is_corresponding":false},{"id":1329672,"name":"Felipe Baeza‐Lehnert","orcid":"0000-0001-8026-2147","position":9,"is_corresponding":false},{"id":832285,"name":"Iván Ruminot","orcid":"0000-0002-3985-384X","position":10,"is_corresponding":false},{"id":1330072,"name":"Carlos Alvarez-Navarro","orcid":null,"position":11,"is_corresponding":false},{"id":1329673,"name":"Alejandro San Martín","orcid":"0000-0002-5608-5117","position":12,"is_corresponding":false},{"id":846672,"name":"Bruno Weber","orcid":"0000-0002-9089-0689","position":13,"is_corresponding":false},{"id":1329674,"name":"Pamela Y. Sandoval","orcid":"0000-0002-6514-3980","position":14,"is_corresponding":false},{"id":1329675,"name":"L. Felipe Barros","orcid":"0000-0002-6623-4833","position":15,"is_corresponding":false},{"id":1330065,"name":"Daniela Rauseo","orcid":null,"position":0,"is_corresponding":true}],"reference_count":42,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:07:57.049469Z","pmid":"39071354","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}