{"doi":"10.1101/2024.07.15.603582","title":"Effect of Voltage-Gated K <sup>+</sup> Channel Inhibition by 4-aminopyridine in Spinal Cord Injury Recovery in Zebrafish","abstract":"Abstract Spinal cord injury (SCI) affects between 250,000 to 500,000 individuals annually. After the initial injury, a delayed secondary cascade of cellular responses occurs causing progressive degeneration and permanent disability. One part of this secondary process is disturbance of ionic homeostasis. The K + channel blocker, 4-aminopyridine (4-AP), is used clinically to alleviate symptoms of multiple sclerosis (MS). Several ongoing studies are being conducted to explore additional areas where 4-AP may have an effect, including stroke, traumatic brain injury, and nervous system recovery after SCI. The goal of our study was to determine whether 4-AP affects recovery from SCI in zebrafish ( Danio rerio ). Using the transgenic line Tg(gfap:EGFP) , we created a spinal transection and tracked swim recovery. We found that constant treatment with 10 µM 4-AP increases swimming distance 40%. Live imaging demonstrated that treatment with 4-AP increases radial glial cells bridging at the site of injury in the presence of 4-AP. We conclude that 10 µM 4-AP is pro-regenerative after SCI. Significance Statement In this study, we found that 4-AP can enhance locomotor recovery in zebrafish after spinal cord injury. Our findings indicate that inhibition of K + channels with 4-AP may promote glial remodeling that is pro-regenerative.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":488740,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9616,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":486079,"name":"K Mruk","orcid":"0000-0003-4560-4951","position":1,"is_corresponding":false},{"id":1333315,"name":"Payge M Hoffman","orcid":"0000-0002-2309-472X","position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":null,"created_at":"2026-07-19T02:08:19.655720Z","pmid":"39071260","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}