{"doi":"10.1101/2024.07.02.601761","title":"A Biparatopic Intrabody Renders Vero Cells Impervious to Ricin Intoxication","abstract":"Abstract Expression of camelid-derived, single-domain antibodies (V H Hs) within the cytoplasm of mammalian cells as “intrabodies” has opened-up novel avenues for medical countermeasures against fast-acting biothreat agents. In this report, we describe a heterodimeric intrabody that renders Vero cells virtually impervious to ricin toxin (RT), a potent Category B ribosome-inactivating protein (RIP). The intrabody consists of two structurally defined V H Hs that target distinct epitopes on RT’s enzymatic subunit (RTA): V9E1 targets RTA’s P-stalk recruitment site, and V2A11 targets RTA’s active site. Resistance to RT conferred by the biparatopic V H H construct far exceeded that of either of the V H Hs alone and effectively inhibited all measurable RT-induced cytotoxicty in vitro . We propose that targeted delivery of bispecific intrabodies to lung tissues may represent a novel means to shield the airways from the effects of inhalational RT exposure.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":491312,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9535,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":430649,"name":"David J. Vance","orcid":"0000-0002-9913-7722","position":1,"is_corresponding":false},{"id":1336923,"name":"Renji Song","orcid":null,"position":2,"is_corresponding":false},{"id":45735,"name":"Nicholas J. Mantis","orcid":"0000-0002-5083-8640","position":3,"is_corresponding":false},{"id":918832,"name":"Timothy F. Czajka","orcid":"0000-0003-3005-8564","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T02:08:41.211111Z","pmid":"39005371","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}