{"doi":"10.1101/2024.06.27.600657","title":"Cryo-electron tomography pipeline for plasma membranes","abstract":"Cryo-electron tomography (cryoET) provides sub-nanometer protein structure within the dense cellular environment. Existing sample preparation methods are insufficient at accessing the plasma membrane and its associated proteins. Here, we present a correlative cryo-electron tomography pipeline optimally suited to image large ultra-thin areas of isolated basal and apical plasma membranes. The pipeline allows for angstrom-scale structure determination with sub-tomogram averaging and employs a genetically-encodable rapid chemically-induced electron microscopy visible tag for marking specific proteins within the complex cell environment. The pipeline provides fast, efficient, distributable, low-cost sample preparation and enables targeted structural studies of identified proteins at the plasma membrane of cells.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":485610,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9416,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":972090,"name":"Dennis J. Michalak","orcid":"0000-0002-0514-4981","position":1,"is_corresponding":false},{"id":402898,"name":"Kem A. Sochacki","orcid":"0000-0003-1160-4558","position":2,"is_corresponding":false},{"id":1329118,"name":"Prasanthi Kunamaneni","orcid":null,"position":3,"is_corresponding":false},{"id":558072,"name":"Marco A. Alfonzo‐Méndez","orcid":null,"position":4,"is_corresponding":false},{"id":1328609,"name":"Andreas M. Arnold","orcid":"0000-0001-9236-1775","position":5,"is_corresponding":false},{"id":716658,"name":"Marie‐Paule Strub","orcid":"0000-0003-4840-1893","position":6,"is_corresponding":false},{"id":258061,"name":"Jenny E. Hinshaw","orcid":"0000-0002-5130-5061","position":7,"is_corresponding":false},{"id":402899,"name":"Justin W. Taraska","orcid":"0000-0001-5355-9535","position":8,"is_corresponding":false},{"id":335939,"name":"Willy W. Sun","orcid":null,"position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:07:52.536246Z","pmid":"39372776","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}