{"doi":"10.1101/2024.06.25.600597","title":"Intraperitoneal activation of myeloid cells clears ascites and reveals IL27-dependent regression of metastatic ovarian cancer","abstract":"Abstract Patients with metastatic ovarian cancer (OvCa) have a 5-year survival rate of less than 30% due to persisting dissemination of chemoresistant cells in the peritoneal fluid and the immunosuppressive microenvironment in the peritoneal cavity. Here, we report that intraperitoneal administration of β-glucan and IFNγ (BI) induced robust tumor regression in clinically relevant models of metastatic OvCa. BI induced tumor regression by controlling fluid tumor burden and activating localized antitumor immunity. β-glucan alone cleared ascites and eliminated fluid tumor cells by inducing intraperitoneal clotting in the fluid and Dectin-1-Syk-dependent NETosis in the omentum. In omentum tumors, BI expanded a novel subset of immunostimulatory IL27+ macrophages and neutralizing IL27 impaired BI efficacy in vivo . Moreover, BI directly induced IL27 secretion in macrophages where single agent treatment did not. Finally, BI extended mouse survival in a chemoresistant model and significantly improved chemotherapy response in a chemo-sensitive model. In summary, we propose a new therapeutic strategy for the treatment of metastatic OvCa.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":491279,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9533,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1209852,"name":"Taito Miyamoto","orcid":"0000-0001-7453-1611","position":1,"is_corresponding":false},{"id":324654,"name":"Bryan Manning","orcid":null,"position":2,"is_corresponding":false},{"id":840205,"name":"Gauri Mirji","orcid":"0000-0001-6439-8204","position":3,"is_corresponding":false},{"id":258868,"name":"Alessio Ugolini","orcid":"0000-0002-5970-9693","position":4,"is_corresponding":false},{"id":443264,"name":"Toshitha Kannan","orcid":"0009-0007-7775-6585","position":5,"is_corresponding":false},{"id":1228318,"name":"Kohei Hamada","orcid":"0009-0003-0354-7722","position":6,"is_corresponding":false},{"id":271174,"name":"Yanfang Peipei Zhu","orcid":"0000-0001-6908-0860","position":7,"is_corresponding":false},{"id":321609,"name":"Daniel T. Claiborne","orcid":"0000-0002-2091-1456","position":8,"is_corresponding":false},{"id":1329634,"name":"Lu Huang","orcid":"0009-0005-2530-569X","position":9,"is_corresponding":false},{"id":231036,"name":"Rugang Zhang","orcid":"0000-0002-7255-2360","position":10,"is_corresponding":false},{"id":245075,"name":"Yulia Nefedova","orcid":"0000-0002-5121-9294","position":11,"is_corresponding":false},{"id":245076,"name":"Andrew V. Kossenkov","orcid":"0000-0002-1536-0418","position":12,"is_corresponding":false},{"id":258870,"name":"Filippo Veglia","orcid":"0000-0001-6710-1584","position":13,"is_corresponding":false},{"id":686190,"name":"Rahul Shinde","orcid":"0000-0001-8767-5032","position":14,"is_corresponding":false},{"id":906998,"name":"Nan Zhang","orcid":"0009-0001-6336-7534","position":15,"is_corresponding":false},{"id":1228317,"name":"Brennah Murphy","orcid":"0000-0003-3698-6513","position":0,"is_corresponding":true}],"reference_count":91,"raw_metadata":null,"created_at":"2026-07-19T02:08:41.211111Z","pmid":"38979222","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}