{"doi":"10.1101/2024.06.18.599456","title":"Glutamate, GABA, and dense-core vesicle secretion regulate predatory feeding in the nematode\n                  <i>Pristionchus pacificus</i>","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Nematodes are one of the most diverse groups of organisms, found in various environments, and exhibit various feeding behaviours.\n                  <jats:italic>Pristionchus pacificus</jats:italic>\n                  displays two types of feeding behaviours: predatory and bacterial feeding. Previously, we showed that the neurotransmitter serotonin plays important roles in regulating predatory feeding in\n                  <jats:italic>P. pacificus.</jats:italic>\n                  However, the role of other neurotransmitters in predatory feeding remains unclear. Using the CRISPR/Cas9 system, we generated mutants of the dense-core vesicle (DCV)-based neuropeptide secretion system and genes related to seven neurotransmitters. Predatory assays revealed that the vesicular glutamate transporter\n                  <jats:italic>Ppa-</jats:italic>\n                  EAT-4, glutamic acid decarboxylase\n                  <jats:italic>Ppa-</jats:italic>\n                  UNC-25 which is involved in GABA synthesis, and the calcium-dependent activator protein for DCV secretion\n                  <jats:italic>Ppa-</jats:italic>\n                  UNC-31, play roles in predatory feeding behaviour. We assessed the pharyngeal movement necessary for predation as well as locomotion rate in these mutants. While the\n                  <jats:italic>Ppa-eat-4</jats:italic>\n                  and\n                  <jats:italic>Ppa-unc-31</jats:italic>\n                  mutants decreased predation movement, the\n                  <jats:italic>Ppa-unc-25</jats:italic>\n                  mutant showed a reduction only in bacterial events compared to wild type animals. Additionally,\n                  <jats:italic>Ppa-unc-25</jats:italic>\n                  and\n                  <jats:italic>Ppa-unc-31</jats:italic>\n                  decreased in motor movement, potentially reducing predation efficiency. Together, these results suggest that glutamate, GABA and DCV secretion modulate feeding behaviours in\n                  <jats:italic>P. pacificus</jats:italic>\n                  . Our mutant collection of neurotransmitter-related genes will be useful for future analysis of neurobiology and behavioural evolution.\n                </jats:p>","journal":null,"year":null,"id":649925,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":714352,"name":"Andrew Zhang","orcid":"0000-0001-9753-2916","position":1,"is_corresponding":false},{"id":1007684,"name":"Takahiro Chihara","orcid":"0000-0001-9989-3619","position":2,"is_corresponding":false},{"id":310676,"name":"Ralf J. Sommer","orcid":"0000-0003-1503-7749","position":3,"is_corresponding":false},{"id":1081764,"name":"Misako Okumura","orcid":"0000-0003-3162-0416","position":4,"is_corresponding":false},{"id":1082182,"name":"Ageha Onodera","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Glutamate, GABA, and dense-core vesicle secretion regulate predatory feeding in the nematode\n                  <i>Pristionchus pacificus</i>","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>\n                  Nematodes are one of the most diverse groups of organisms, found in various environments, and exhibit various feeding behaviours.\n                  <jats:italic>Pristionchus pacificus</jats:italic>\n                  displays two types of feeding behaviours: predatory and bacterial feeding. Previously, we showed that the neurotransmitter serotonin plays important roles in regulating predatory feeding in\n                  <jats:italic>P. pacificus.</jats:italic>\n                  However, the role of other neurotransmitters in predatory feeding remains unclear. Using the CRISPR/Cas9 system, we generated mutants of the dense-core vesicle (DCV)-based neuropeptide secretion system and genes related to seven neurotransmitters. Predatory assays revealed that the vesicular glutamate transporter\n                  <jats:italic>Ppa-</jats:italic>\n                  EAT-4, glutamic acid decarboxylase\n                  <jats:italic>Ppa-</jats:italic>\n                  UNC-25 which is involved in GABA synthesis, and the calcium-dependent activator protein for DCV secretion\n                  <jats:italic>Ppa-</jats:italic>\n                  UNC-31, play roles in predatory feeding behaviour. We assessed the pharyngeal movement necessary for predation as well as locomotion rate in these mutants. While the\n                  <jats:italic>Ppa-eat-4</jats:italic>\n                  and\n                  <jats:italic>Ppa-unc-31</jats:italic>\n                  mutants decreased predation movement, the\n                  <jats:italic>Ppa-unc-25</jats:italic>\n                  mutant showed a reduction only in bacterial events compared to wild type animals. Additionally,\n                  <jats:italic>Ppa-unc-25</jats:italic>\n                  and\n                  <jats:italic>Ppa-unc-31</jats:italic>\n                  decreased in motor movement, potentially reducing predation efficiency. Together, these results suggest that glutamate, GABA and DCV secretion modulate feeding behaviours in\n                  <jats:italic>P. pacificus</jats:italic>\n                  . Our mutant collection of neurotransmitter-related genes will be useful for future analysis of neurobiology and behavioural evolution.\n                </jats:p>","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19162232","pmcid":null,"openalex_id":"https://openalex.org/W4399929588","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2024,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2024/06/22/2024.06.18.599456.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2024/06/22/2024.06.18.599456.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2024.06.18.599456","host_type":"publisher"},{"url":"http://dx.doi.org/10.1101/2024.06.18.599456","host_type":"repository"},{"url":"https://doi.org/10.1101/2024.06.18.599456","host_type":"Unpaywall"}],"fields_of_study":["Nematode management and characterization studies","Parasite Biology and Host Interactions"],"mesh_terms":[],"keywords":["Nematode","Secretion","Biology","Glutamate receptor","Predation","Zoology","Ecology","Endocrinology","Receptor","Biochemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T04:32:09.525359Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}