{"doi":"10.1101/2024.06.14.599074","title":"A higher order PUF complex is central to regulation of <i>C. elegans</i> germline stem cells","abstract":"Abstract PUF RNA-binding proteins are broadly conserved stem cell regulators. Nematode PUF proteins maintain germline stem cells (GSCs) and, with key partner proteins, repress differentiation mRNAs, including gld-1 . Here we report that PUF protein FBF-2 and its partner LST-1 form a ternary complex that represses gld-1 via a pair of adjacent FBF-2 binding elements (FBEs) in its 3’UTR. One LST-1 molecule links two FBF-2 molecules via motifs in the LST-1 intrinsically-disordered region; the gld-1 FBE pair includes a well-established ‘canonical’ FBE and a newly-identified noncanonical FBE. Remarkably, this FBE pair drives both full RNA repression in GSCs and full RNA activation upon differentiation. Discovery of the LST-1–FBF-2 ternary complex, the gld-1 adjacent FBEs, and their in vivo significance predicts an expanded regulatory repertoire of different assemblies of PUF-partner complexes in nematode germline stem cells. It also suggests analogous PUF controls may await discovery in other biological contexts and organisms.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":491183,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9561,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":438899,"name":"Sarah L. Crittenden","orcid":null,"position":1,"is_corresponding":false},{"id":1145286,"name":"Brian H. Carrick","orcid":"0000-0003-0139-3582","position":2,"is_corresponding":false},{"id":559409,"name":"Lucas Dillard","orcid":"0000-0003-4325-8055","position":3,"is_corresponding":false},{"id":1096736,"name":"Stephany J. Costa Dos Santos","orcid":"0000-0002-4147-6756","position":4,"is_corresponding":false},{"id":258055,"name":"Venkata P. Dandey","orcid":"0000-0001-7283-2664","position":5,"is_corresponding":false},{"id":860526,"name":"Robert Dutcher","orcid":"0000-0002-5144-2111","position":6,"is_corresponding":false},{"id":909044,"name":"Elizabeth Viverette","orcid":"0000-0002-5173-5838","position":7,"is_corresponding":false},{"id":519643,"name":"Robert N. Wine","orcid":"0009-0009-3242-5699","position":8,"is_corresponding":false},{"id":1295939,"name":"Jennifer Woodworth","orcid":null,"position":9,"is_corresponding":false},{"id":279176,"name":"Zachary T. Campbell","orcid":"0000-0002-3768-6996","position":10,"is_corresponding":false},{"id":255523,"name":"Marvin Wickens","orcid":"0000-0002-0593-5740","position":11,"is_corresponding":false},{"id":227365,"name":"Mario J. Borgnia","orcid":"0000-0001-9159-1413","position":12,"is_corresponding":false},{"id":438022,"name":"Judith Kimble","orcid":"0000-0001-5622-2073","position":13,"is_corresponding":false},{"id":458362,"name":"Traci M. Tanaka Hall","orcid":"0000-0001-6166-3009","position":15,"is_corresponding":false},{"id":519642,"name":"Chen Qiu","orcid":"0000-0003-4294-7537","position":0,"is_corresponding":true}],"reference_count":80,"raw_metadata":null,"created_at":"2026-07-19T02:08:41.211111Z","pmid":"38915480","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}