{"doi":"10.1101/2024.05.09.24307112","title":"Simultaneous co-circulation of two genotypes of dengue virus serotype 3 causing a large outbreak in Sri Lanka in year 2023","abstract":"Abstract As many other countries, Sri Lanka experienced a marked rise in the number of dengue cases in 2023, with an unusual pattern of disease epidemiology. This rise coincided with the emergence of dengue virus (DENV) serotype 3 in Sri Lanka as the predominant serotype after 2009. Interestingly, a discrepancy between NS1 rapid antigen test positivity and quantitative real time PCR positivity was observed, with 50% of NS1 positive samples being negative by molecular diagnostics. Following sequencing of the DENV-3 strains in 2023, we identified two DENV-3 genotypes (I and III) co-circulating. While DENV-3 genotype III was detected by the modified CDC DENV-3 primers, genotype I evaded detection due to key mutations at forward and reverse primer binding sites. The co-circulation of multiple genotypes associated with an increase in cases highlights the importance of continuous surveillance of DENVs to identify mutations resulting in non-detection by diagnostics and differences in virulence. One-sentence summary line Co-circulation of two genotypes of the dengue virus serotype-3 (I and III) were responsible for the large dengue outbreak in Sri Lanka in 2023, with genotype I not been detected by certain PCR primers.","journal":"medRxiv","year":2024,"id":488480,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9492,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1254359,"name":"Bhagya Senadheera","orcid":null,"position":1,"is_corresponding":false},{"id":804530,"name":"Heshan Kuruppu","orcid":"0009-0005-8745-8202","position":2,"is_corresponding":false},{"id":778394,"name":"Tibutius T. P. Jayadas","orcid":"0000-0003-3041-7280","position":3,"is_corresponding":false},{"id":479262,"name":"Laksiri Gomes","orcid":"0000-0002-2336-7919","position":4,"is_corresponding":false},{"id":1253760,"name":"D.M.S.H.K. Ranasinghe","orcid":"0000-0001-5151-8908","position":5,"is_corresponding":false},{"id":1133832,"name":"Farha Bary","orcid":null,"position":6,"is_corresponding":false},{"id":495842,"name":"Ananda Wijewickrama","orcid":"0000-0003-2723-1813","position":7,"is_corresponding":false},{"id":1333498,"name":"Sully Márquez Aguilar","orcid":null,"position":8,"is_corresponding":false},{"id":377891,"name":"Shannon N. Bennett","orcid":null,"position":9,"is_corresponding":false},{"id":778393,"name":"Chandima Jeewandara","orcid":"0000-0001-6830-5175","position":10,"is_corresponding":false},{"id":479263,"name":"Gathsaurie Neelika Malavige","orcid":"0000-0001-9201-0449","position":11,"is_corresponding":false},{"id":1172601,"name":"Dinuka Ariyaratne","orcid":"0000-0002-9843-5795","position":0,"is_corresponding":true}],"reference_count":24,"raw_metadata":null,"created_at":"2026-07-19T02:08:19.655720Z","pmid":"38766092","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}