{"doi":"10.1101/2024.04.21.590446","title":"Nanodisc reconstitution and characterization of amyloid-β precursor protein C99","abstract":"ABSTRACT Amyloid precursor protein (APP) plays a pivotal role in the pathology of Alzheimer’s disease. Since the fragmentation of the membrane-bound APP that results in the production of amyloid-beta peptides is the starting point for amyloid toxicity in AD, it is important to investigate the structure and dynamics of APP in a near-native lipid-bilayer environment. However, the reconstitution of APP into a stable/suitable membrane-mimicking lipid environment is a challenging task. In this study, the 99-residue C-terminal domain of APP is successfully reconstituted into polymer nanodiscs and characterized using size-exclusion chromatography, mass spectrometry, solution NMR, and magic-angle spinning solid-state NMR. In addition, the feasibility of using lipid-solubilizing polymers for isolating and characterizing APP in native E. coli membrane environment is demonstrated.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":490745,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9576,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1051067,"name":"Gaurav Sharma","orcid":"0000-0002-0369-6686","position":1,"is_corresponding":false},{"id":1335958,"name":"Volodymyr M. Hiiuk","orcid":"0000-0003-3665-6290","position":2,"is_corresponding":false},{"id":696683,"name":"Jochem Struppe","orcid":"0000-0001-9001-5991","position":3,"is_corresponding":false},{"id":339047,"name":"Pavel Nagorny","orcid":"0000-0002-7043-984X","position":4,"is_corresponding":false},{"id":256238,"name":"Magdalena I. Ivanova","orcid":"0000-0002-7290-3327","position":5,"is_corresponding":false},{"id":256242,"name":"Ayyalusamy Ramamoorthy","orcid":"0000-0003-1964-1900","position":6,"is_corresponding":false},{"id":473601,"name":"Bankala Krishnarjuna","orcid":"0000-0002-4575-0011","position":0,"is_corresponding":true}],"reference_count":69,"raw_metadata":null,"created_at":"2026-07-19T02:08:37.162700Z","pmid":"38659865","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}