{"doi":"10.1101/2024.04.17.588758","title":"Polycystins recruit cargo to distinct ciliary extracellular vesicle subtypes","abstract":"Summary Therapeutic use of tiny extracellular vesicles (EVs) requires understanding cargo loading mechanisms. Here, we used a modular proximity label approach to identify EV cargo associated with the transient potential channel (TRP) polycystin PKD-2 of C. elegans . Polycystins are conserved receptor-TRP channel proteins affecting cilium function; dysfunction causes polycystic kidney disease in humans and mating deficits in C. elegans . Polycystin-2 EV localization is conserved from algae to humans, hinting at an ancient and unknown function. We discovered that polycystins associate with and direct specific cargo to EVs: channel-like PACL-1, dorsal and ventral membrane C-type lectins PAMLs, and conserved tumor necrosis-associated factor (TRAF) signaling adaptors TRF-1 and TRF-2. Loading of these components relied on polycystin-1 LOV-1. Our modular EV-TurboID approach can be applied in both cell– and tissue-specific manners to define the composition of distinct EV subtypes, addressing a major challenge of the EV field.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":490705,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9573,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1336252,"name":"Elizabeth desRanleau","orcid":null,"position":2,"is_corresponding":false},{"id":962505,"name":"Katherine C. Jacobs","orcid":"0000-0001-6828-0828","position":3,"is_corresponding":false},{"id":309447,"name":"Joshua Saul","orcid":null,"position":4,"is_corresponding":false},{"id":429884,"name":"Jonathon D. Walsh","orcid":"0000-0002-4277-7657","position":5,"is_corresponding":false},{"id":274538,"name":"Juan Wang","orcid":"0000-0003-1992-0539","position":6,"is_corresponding":false},{"id":345359,"name":"Maureen M. Barr","orcid":"0000-0003-4483-2952","position":7,"is_corresponding":false},{"id":353205,"name":"Inna A. Nikonorova","orcid":"0000-0001-8498-848X","position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":null,"created_at":"2026-07-19T02:08:37.162700Z","pmid":"38659811","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}