{"doi":"10.1101/2024.03.28.587232","title":"CRISPR/Cas9 gene editing in <i>Drosophila</i> via visual selection in a summer classroom","abstract":"Abstract CRISPR/Cas9 methods are a powerful in vivo approach to edit the genome of Drosophila melanogaster . To convert existing Drosophila GAL4 lines to LexA driver lines in a secondary school classroom setting, we applied the CRISPR-based genetic approach to a collection of Gal4 ‘driver’ lines. The integration of the yellow + coat color marker into homology-assisted CRISPR knock-in (HACK) enabled visual selection of Gal4-to-LexA conversions using brightfield stereo-microscopy available in a broader set of standard classrooms. Here, we report the successful conversion of eleven Gal4 lines with expression in neuropeptide-expressing cells into corresponding, novel LexA drivers. The conversion was confirmed by LexA- and Gal4- specific GFP reporter gene expression. This curriculum was successfully implemented in a summer course running 16 hours/week for seven weeks. The modularity, flexibility, and compactness of this course should enable development of similar classes in secondary schools and undergraduate curricula, to provide opportunities for experience-based science instruction, and university-secondary school collaborations that simultaneously fulfill research needs in the community of science.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":496113,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9569,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1342756,"name":"Valentina Zhang","orcid":"0009-0008-9857-4083","position":1,"is_corresponding":false},{"id":1343024,"name":"Jenna Wang","orcid":null,"position":2,"is_corresponding":false},{"id":1012039,"name":"Clara Gulick","orcid":null,"position":3,"is_corresponding":false},{"id":352345,"name":"Madeleine Laws","orcid":null,"position":4,"is_corresponding":false},{"id":1012038,"name":"Arjun Rajan","orcid":null,"position":5,"is_corresponding":false},{"id":901322,"name":"Nicole Lantz","orcid":null,"position":6,"is_corresponding":false},{"id":1343025,"name":"Ayla Asgarova","orcid":null,"position":7,"is_corresponding":false},{"id":1343026,"name":"Lillian Dai","orcid":null,"position":8,"is_corresponding":false},{"id":1343027,"name":"Kristian Garcia","orcid":null,"position":9,"is_corresponding":false},{"id":507633,"name":"Charlene Kim","orcid":null,"position":10,"is_corresponding":false},{"id":663821,"name":"Michelle Li","orcid":"0000-0002-7545-0992","position":11,"is_corresponding":false},{"id":1343028,"name":"Patricio Ordonez-Acosta","orcid":null,"position":12,"is_corresponding":false},{"id":1343029,"name":"Dongshen Peng","orcid":null,"position":13,"is_corresponding":false},{"id":1343030,"name":"Henry Shull","orcid":null,"position":14,"is_corresponding":false},{"id":1343031,"name":"Lauren Tse","orcid":null,"position":15,"is_corresponding":false},{"id":1343032,"name":"Yixang Wang","orcid":null,"position":16,"is_corresponding":false},{"id":430774,"name":"Wenxin Yu","orcid":"0000-0001-6316-0309","position":17,"is_corresponding":false},{"id":1343033,"name":"Zee Zhou","orcid":null,"position":18,"is_corresponding":false},{"id":901321,"name":"Anne E Rankin","orcid":null,"position":19,"is_corresponding":false},{"id":900722,"name":"Sangbin Park","orcid":"0000-0002-2304-1898","position":20,"is_corresponding":false},{"id":44518,"name":"Seung K. Kim","orcid":"0000-0001-6135-7810","position":21,"is_corresponding":false},{"id":838965,"name":"Lutz Kockel","orcid":"0000-0003-4631-8594","position":0,"is_corresponding":true}],"reference_count":18,"raw_metadata":null,"created_at":"2026-07-19T02:09:23.333150Z","pmid":"38585736","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}