{"doi":"10.1101/2024.03.19.585100","title":"HOCl-producing Electrochemical Bandage is Active in Murine Polymicrobial Wound Infection","abstract":"Abstract Wound infections, exacerbated by the prevalence of antibiotic-resistant bacterial pathogens, necessitate innovative antimicrobial approaches. Polymicrobial infections, often involving Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA), present formidable challenges due to biofilm formation and antibiotic resistance. Hypochlorous acid (HOCl), a potent antimicrobial agent produced naturally by the immune system, holds promise as an alternative therapy. An electrochemical bandage (e-bandage) that generates HOCl in situ was evaluated for treatment of murine wound biofilm infections containing both MRSA and P. aeruginosa with “difficult-to-treat” resistance. Previously, the HOCl-producing e-bandage was shown to reduce wound biofilms containing P. aeruginosa alone. Compared to non-polarized e-bandage (no HOCl production) and Tegaderm only controls, the polarized e-bandages reduced bacterial loads in wounds infected with MRSA plus P. aeruginosa (MRSA: vs Tegaderm only – 1.4 log 10 CFU/g, p = 0.0015, vs. non-polarized – 1.1 log 10 CFU/g, p = 0.026. P. aeruginosa : vs Tegaderm only – 1.6 log 10 CFU/g, p = 0.0015, vs non-polarized – 1.6 log 10 CFU/g, p = 0.0032), and MRSA alone (vs Tegaderm only – 1.3 log 10 CFU/g, p = 0.0048, vs. non-polarized – 1.1 log 10 CFU/g, p = 0.0048), without compromising wound healing or causing tissue toxicity. Addition of systemic antibiotics did not enhance the antimicrobial efficacy of e-bandages, highlighting their potential as standalone therapies. This study provides additional evidence for the HOCl-producing e-bandage as a novel antimicrobial strategy for managing wound infections, including in the context of antibiotic resistance and polymicrobial infections.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":495497,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9568,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1169929,"name":"Ibrahim Bozyel","orcid":"0000-0002-2841-7890","position":1,"is_corresponding":false},{"id":1170425,"name":"Christina Koscianski","orcid":null,"position":2,"is_corresponding":false},{"id":1169930,"name":"Dilara Ozdemir","orcid":"0000-0003-1304-3053","position":3,"is_corresponding":false},{"id":907303,"name":"Melissa J. Karau","orcid":"0000-0001-6021-4033","position":4,"is_corresponding":false},{"id":1287021,"name":"Luz Cuello","orcid":"0009-0001-0000-9916","position":5,"is_corresponding":false},{"id":920440,"name":"Md Monzurul Islam Anoy","orcid":"0000-0001-6876-2632","position":6,"is_corresponding":false},{"id":911904,"name":"Suzanne Gelston","orcid":null,"position":7,"is_corresponding":false},{"id":264773,"name":"Audrey N. Schuetz","orcid":"0000-0002-5837-270X","position":8,"is_corresponding":false},{"id":418597,"name":"Kerryl E. Greenwood‐Quaintance","orcid":"0000-0003-3760-4310","position":9,"is_corresponding":false},{"id":709240,"name":"Jayawant N. Mandrekar","orcid":"0000-0002-9718-4082","position":10,"is_corresponding":false},{"id":418598,"name":"Haluk Beyenal","orcid":"0000-0003-3931-0244","position":11,"is_corresponding":false},{"id":232756,"name":"Robin Patel","orcid":"0000-0001-6344-4141","position":12,"is_corresponding":false},{"id":424298,"name":"Derek Fleming","orcid":"0000-0002-0054-904X","position":0,"is_corresponding":true}],"reference_count":28,"raw_metadata":null,"created_at":"2026-07-19T02:09:19.627139Z","pmid":"38562889","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}