{"doi":"10.1101/2024.03.13.581057","title":"Bioluminescence Imaging of Potassium Ion Using a Sensory Luciferin and an Engineered Luciferase","abstract":"ABSTRACT Bioluminescent indicators are power tools for studying dynamic biological processes. In this study, we present the generation of novel bioluminescent indicators by modifying the luciferin molecule with an analyte-binding moiety. Specifically, we have successfully developed the first bioluminescent indicator for potassium ions (K + ), which are critical electrolytes in biological systems. Our approach involved the design and synthesis of a K + -binding luciferin named potassiorin. Additionally, we engineered a luciferase enzyme called BRIPO (bioluminescent red indicator for potassium) to work synergistically with potassiorin, resulting in optimized K + -dependent bioluminescence responses. Through extensive validation in cell lines, primary neurons, and live mice, we demonstrated the efficacy of this new tool for detecting K + . Our research demonstrates an innovative concept of incorporating sensory moieties into luciferins to modulate luciferase activity. This approach has great potential for developing a wide range of bioluminescent indicators, advancing bioluminescence imaging (BLI), and enabling the study of various analytes in biological systems. Abstract Figure","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":490323,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9525,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1103043,"name":"Ying Xiong","orcid":"0009-0002-3581-2845","position":1,"is_corresponding":false},{"id":1335708,"name":"Ranganayakulu Sunnapu","orcid":null,"position":2,"is_corresponding":false},{"id":860721,"name":"Yiyu Zhang","orcid":"0000-0001-8636-2827","position":3,"is_corresponding":false},{"id":860720,"name":"Xiaodong Tian","orcid":"0000-0001-9590-9943","position":4,"is_corresponding":false},{"id":336191,"name":"Hui‐wang Ai","orcid":"0000-0002-5664-3270","position":5,"is_corresponding":false},{"id":762222,"name":"Shengyu Zhao","orcid":"0000-0002-2501-8464","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-19T02:08:32.775003Z","pmid":"38559024","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}