{"doi":"10.1101/2024.03.09.584243","title":"Three-dimensional spatio-angular fluorescence microscopy with a polarized dual-view inverted selective-plane illumination microscope (pol-diSPIM)","abstract":"Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations, but techniques for recovering three-dimensional orientations and positions of fluorescent ensembles are limited. We report a polarized dual-view light-sheet system for determining the three-dimensional orientations and diffraction-limited positions of ensembles of fluorescent dipoles that label biological structures, and we share a set of visualization, histogram, and profiling tools for interpreting these positions and orientations. We model our samples, their excitation, and their detection using coarse-grained representations we call orientation distribution functions (ODFs). We apply ODFs to create physics-informed models of image formation with spatio-angular point-spread and transfer functions. We use theory and experiment to conclude that light-sheet tilting is a necessary part of our design for recovering all three-dimensional orientations. We use our system to extend known two-dimensional results to three dimensions in FM1-43-labelled giant unilamellar vesicles, fast-scarlet-labelled cellulose in xylem cells, and phalloidin-labelled actin in U2OS cells. Additionally, we observe phalloidin-labelled actin in mouse fibroblasts grown on grids of labelled nanowires and identify correlations between local actin alignment and global cell-scale orientation, indicating cellular coordination across length scales.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":486980,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9558,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":355125,"name":"Min Guo","orcid":"0000-0002-2093-8771","position":1,"is_corresponding":false},{"id":246595,"name":"Yijun Su","orcid":"0009-0001-4630-7863","position":2,"is_corresponding":false},{"id":246601,"name":"Jiji Chen","orcid":"0000-0002-4426-3035","position":3,"is_corresponding":false},{"id":246614,"name":"Yicong Wu","orcid":"0000-0002-8819-7527","position":4,"is_corresponding":false},{"id":1331029,"name":"Junyu Liu","orcid":"0000-0003-4276-9531","position":5,"is_corresponding":false},{"id":1073055,"name":"Atharva Agashe","orcid":"0009-0002-1241-2160","position":6,"is_corresponding":false},{"id":632378,"name":"Robert Fischer","orcid":"0000-0003-0650-2696","position":7,"is_corresponding":false},{"id":558606,"name":"Shalin B. Mehta","orcid":"0000-0002-2542-3582","position":8,"is_corresponding":false},{"id":564372,"name":"Abhishek Kumar","orcid":"0000-0002-4961-6469","position":9,"is_corresponding":false},{"id":1331030,"name":"Tobias I. Baskin","orcid":"0000-0001-5189-3310","position":10,"is_corresponding":false},{"id":1331378,"name":"Valentin Jamouille","orcid":null,"position":11,"is_corresponding":false},{"id":246613,"name":"Huafeng Liu","orcid":"0000-0002-9737-9437","position":12,"is_corresponding":false},{"id":543942,"name":"Vinay Swaminathan","orcid":"0000-0002-9946-810X","position":13,"is_corresponding":false},{"id":461592,"name":"Amrinder S. Nain","orcid":"0000-0002-9757-2341","position":14,"is_corresponding":false},{"id":415896,"name":"Rudolf Oldenbourg","orcid":"0000-0003-1055-8692","position":15,"is_corresponding":false},{"id":246612,"name":"Patrick J. La Rivière","orcid":"0000-0003-3415-9864","position":16,"is_corresponding":false},{"id":246615,"name":"Hari Shroff","orcid":"0000-0003-3613-8215","position":17,"is_corresponding":false},{"id":568915,"name":"Talon Chandler","orcid":"0000-0002-3033-674X","position":0,"is_corresponding":true}],"reference_count":74,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:08:06.013846Z","pmid":"38712306","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}