{"doi":"10.1101/2024.03.03.583222","title":"IPPK-1 and IP6 contribute to ventral nerve cord assembly in <i>C. elegans</i>","abstract":"Abstract Inositol phosphates (IPs) are essential for the development and function of the nervous system. Loss-of-function studies, which demonstrate the importance of specific IP isomers, show their critical role in proper neural tube formation. In this study, we show that inositol pentakisphosphate 2-kinase (IPPK-1), the kinase that phosphorylates IP5 to generate IP6, is involved in assembling the ventral nerve cord (VNC) in C. elegans . We show that mutations in ippk-1 lead to the mispositioning of motor neurons along the VNC of newly hatched larvae. These positioning defects reflect disruption of VNC assembly during embryogenesis, as VNC neuronal progenitors in ippk-1 embryos display a more compact organization after arising on the left and right sides of the embryo, delays in rosette-mediated convergent extension, and defects in cell intercalation. We further show that injection of exogenous IP6 into the gonads of ippk-1 mutants can rescue both embryonic and neuron positioning defects. Our findings indicate that IP isomers, particularly IP6, are important for ventral nerve cord formation in C. elegans . Along with their role in neural tube formation in vertebrates, these results suggests that IP isomers play an ancient role in central nerve cord development. Highlights - ipmk-1 and ippk-1 mutants display neuron position defects in the ventral nerve cord (VNC). - ippk-1 mutants display disorganization in VNC neuronal progenitors during VNC assembly. - IPPK-1 is involved in convergent extension during VNC formation. - Exogenous IP6 rescues larval and embryonic defects in ippk-1 mutants.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":488044,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9561,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1332858,"name":"Tony Roenspies","orcid":null,"position":1,"is_corresponding":false},{"id":367487,"name":"Stéphane Flibotte","orcid":"0000-0001-9613-7666","position":2,"is_corresponding":false},{"id":1281461,"name":"A. Colavita","orcid":"0000-0001-9391-5282","position":3,"is_corresponding":false},{"id":1281460,"name":"Nathaniel Noblett","orcid":"0000-0003-0393-9104","position":0,"is_corresponding":true}],"reference_count":89,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:08:15.008850Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}