{"doi":"10.1101/2024.02.22.581598","title":"Activity of DNA polymerase κ across the genome in human fibroblasts","abstract":"Abstract DNA polymerase κ (Pol κ) is a specialized polymerase that has multiple cellular roles such as translesion DNA synthesis, replicating repetitive sequences, and nucleotide excision repair. We have developed a method for capturing DNA synthesized by Pol κ utilizing a Pol κ - specific substrate, N 2 -(4-ethynylbenzyl)-2′-deoxyguanosine (EBndG). After shearing of the DNA into 200-500bp lengths, the EBndG-containing DNA was covalently bound to biotin using the Cu(I)- catalyzed alkyne–azide cycloaddition reaction, and isolated with streptavidin beads. Isolated DNA was then ligated to adaptors, followed by PCR amplification and next-generation sequencing (NGS) to generate genome-wide repair maps. We have termed this method polymerase κ sequencing (polK-seq). Here we present the human genome maps for pol κ activity in an undamaged cell line. We found that pol κ activity was enhanced in euchromatin regions, the promoter of genes, and in DNA that is replicated early in S-phase.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":490123,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9628,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":533676,"name":"Dongxiao Sun","orcid":"0000-0003-4902-4292","position":1,"is_corresponding":false},{"id":1335358,"name":"Thomas E. Spratt","orcid":"0000-0002-6805-3729","position":2,"is_corresponding":false},{"id":1335357,"name":"M. Torres","orcid":"0000-0002-1988-6898","position":0,"is_corresponding":true}],"reference_count":19,"raw_metadata":null,"created_at":"2026-07-19T02:08:32.775003Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}