{"doi":"10.1101/2024.02.15.580492","title":"A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions","abstract":"Abstract The endoplasmic reticulum (ER) is an important regulator of Ca 2+ in cells and dysregulation of ER calcium homeostasis can lead to numerous pathologies. Understanding how various pharmacological and genetic perturbations of ER Ca 2+ homeostasis impacts cellular physiology would likely be facilitated by more quantitative measurements of ER Ca 2+ levels that allow easier comparisons across conditions. Here, we developed a ratiometric version of our original ER-GCaMP probe that allows for more quantitative comparisons of the concentration of Ca 2+ in the ER across cell types and sub-cellular compartments. Using this approach we show that the resting concentration of ER Ca2+ in primary dissociated neurons is substantially lower than that in measured in embryonic fibroblasts.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":484632,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.95,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":383950,"name":"Kirsten Bredvik","orcid":"0000-0003-4613-4808","position":1,"is_corresponding":false},{"id":579642,"name":"Michael B. Hoppa","orcid":"0000-0003-2340-0915","position":2,"is_corresponding":false},{"id":478847,"name":"S. Thomas Hennigan","orcid":"0000-0003-3100-1914","position":3,"is_corresponding":false},{"id":470047,"name":"Timothy A. Brown","orcid":"0000-0001-8941-7678","position":4,"is_corresponding":false},{"id":568496,"name":"Timothy Aidan Ryan","orcid":"0000-0003-2533-9548","position":5,"is_corresponding":false},{"id":704493,"name":"Ryan Farrell","orcid":"0000-0003-4022-8707","position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:07:42.971417Z","pmid":"38405980","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}