{"doi":"10.1101/2024.02.03.578580","title":"IFNγ induces epithelial reprogramming driving CXCL11-mediated T cell migration","abstract":"The cytokine interferon-gamma (IFNγ) plays a multifaceted role in intestinal immune responses ranging from anti-to pro-inflammatory depending on the setting. Here, using a 3D co-culture system based on human intestinal epithelial organoids, we explore the capacity of IFNγ-exposure to reprogram intestinal epithelia and thereby directly modulate lymphocyte responses. IFNγ treatment of organoids led to transcriptional reprogramming, marked by a switch to a pro-inflammatory gene expression profile, including transcriptional upregulation of the chemokines CXCL9, CXCL10, and CXCL11. Proteomic analysis of organoid-conditioned medium post-treatment confirmed chemokine secretion. Furthermore, IFNγ-treatment of organoids led to enhanced T cell migration in a CXCL11-dependent manner without affecting T cell activation status. Taken together, our results suggest a specific role for CXCL11 in T cell recruitment that can be targeted to prevent T cell trafficking to the inflamed intestine.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":485930,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9567,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":888558,"name":"Suze A. Jansen","orcid":"0000-0002-1225-9963","position":1,"is_corresponding":false},{"id":1307100,"name":"Francesca Paolucci","orcid":null,"position":2,"is_corresponding":false},{"id":469984,"name":"Michal Mokrý","orcid":"0000-0002-5298-4852","position":3,"is_corresponding":false},{"id":1286658,"name":"Enric Mocholí","orcid":"0000-0002-6614-3173","position":4,"is_corresponding":false},{"id":297901,"name":"Caroline A. Lindemans","orcid":"0000-0002-3984-5620","position":5,"is_corresponding":false},{"id":1306667,"name":"Paul J. Coffer","orcid":"0000-0001-8775-1939","position":6,"is_corresponding":false},{"id":1287115,"name":"Alessandro Cutilli","orcid":null,"position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":null,"created_at":"2026-07-19T02:07:57.049469Z","pmid":"38370633","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}