{"doi":"10.1101/2024.01.14.575614","title":"Genome scale CRISPR screens identify actin capping proteins as key modulators of therapeutic responses to radiation and immunotherapy","abstract":"Abstract Radiotherapy (RT), is a fundamental treatment for malignant tumors and is used in over half of cancer patients. As radiation can promote anti-tumor immune effects, a promising therapeutic strategy is to combine radiation with immune checkpoint inhibitors (ICIs). However, the genetic determinants that impact therapeutic response in the context of combination therapy with radiation and ICI have not been systematically investigated. To unbiasedly identify the tumor intrinsic genetic factors governing such responses, we performed a set of genome-scale CRISPR screens in melanoma cells for cancer survival in response to low-dose genotoxic radiation treatment, in the context of CD8 T cell co-culture and with anti-PD1 checkpoint blockade antibody. Two actin capping proteins, Capza3 and Capg , emerged as top hits that upon inactivation promote the survival of melanoma cells in such settings. Capza3 and Capg knockouts (KOs) in mouse and human cancer cells display persistent DNA damage due to impaired homology directed repair (HDR); along with increased radiation, chemotherapy, and DNA repair inhibitor sensitivity. However, when cancer cells with these genes inactivated were exposed to sublethal radiation, the persistence of DNA damage promoted activation of the STING pathway, induction of inhibitory CEACAM1 ligand expression and resistance to CD8 T cell killing. Patient cancer genomics analysis reveals an increased mutational burden in patients with inactivating mutations in CAPG and/or CAPZA3 , at levels comparable to other HDR associated genes. There is also a positive correlation between CAPG expression, CD8 T cell tumor infiltration and response to immunotherapy among patient datasets. Our results unveil the critical roles of actin binding proteins for efficient HDR within cancer cells and demonstrate a previously unrecognized regulatory mechanism of therapeutic response to radiation and immunotherapy.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2024,"id":493327,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9556,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":259572,"name":"Paul Renauer","orcid":"0000-0002-8747-0552","position":1,"is_corresponding":false},{"id":342243,"name":"Chuanpeng Dong","orcid":"0000-0001-5068-0495","position":2,"is_corresponding":false},{"id":991368,"name":"Yanzhi Feng","orcid":null,"position":3,"is_corresponding":false},{"id":454811,"name":"Ping Ren","orcid":"0000-0001-8694-8705","position":4,"is_corresponding":false},{"id":861703,"name":"Qianqian Lin","orcid":"0000-0002-9196-7692","position":5,"is_corresponding":false},{"id":259571,"name":"Feifei Zhang","orcid":"0000-0003-3277-445X","position":6,"is_corresponding":false},{"id":1339937,"name":"Narges Tafreshi","orcid":null,"position":7,"is_corresponding":false},{"id":272426,"name":"Peter M. Glazer","orcid":"0000-0003-4525-5560","position":8,"is_corresponding":false},{"id":60778,"name":"Sidi Chen","orcid":"0000-0002-3819-5005","position":9,"is_corresponding":false},{"id":406169,"name":"Nipun Verma","orcid":"0000-0002-9522-6699","position":10,"is_corresponding":false},{"id":1029378,"name":"Xin Shan","orcid":"0000-0002-4735-0935","position":0,"is_corresponding":true}],"reference_count":79,"raw_metadata":null,"created_at":"2026-07-19T02:09:00.148120Z","pmid":"38293095","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}