{"doi":"10.1101/2023.12.29.573615","title":"Identification of SLC25A46 interaction interfaces with mitochondrial membrane fusogens Opa1 and Mfn2","abstract":"Mitochondrial fusion requires the sequential merger of four bilayers to two. The outer-membrane solute carrier protein SLC25A46 interacts with both the outer and inner-membrane dynamin family GTPases Mfn1/2 and Opa1. While SLC25A46 levels are known to affect mitochondrial morphology, how SLC25A46 interacts with Mfn1/2 and Opa1 to regulate membrane fusion is not understood. In this study, we use crosslinking mass-spectrometry and AlphaFold 2 modeling to identify interfaces mediating a SLC25A46 interactions with Opa1 and Mfn2. We reveal that the bundle signaling element of Opa1 interacts with SLC25A46, and present evidence of a Mfn2 interaction involving the SLC25A46 cytosolic face. We validate these newly identified interaction interfaces and show that they play a role in mitochondrial network maintenance.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":393982,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9591,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1162262,"name":"Bridget E Luce","orcid":"0000-0002-0107-5181","position":1,"is_corresponding":false},{"id":648332,"name":"Camila Makhlouta Lugo","orcid":"0000-0003-4655-8096","position":2,"is_corresponding":false},{"id":1162260,"name":"Pusparanee Hakim","orcid":"0000-0002-9018-8179","position":3,"is_corresponding":false},{"id":1162263,"name":"Julie L. McDonald","orcid":"0000-0002-3715-9619","position":4,"is_corresponding":false},{"id":1168779,"name":"Ha Lin Kim","orcid":null,"position":5,"is_corresponding":false},{"id":985739,"name":"Jackeline Ponce","orcid":"0000-0002-3886-9989","position":6,"is_corresponding":false},{"id":231337,"name":"Beatrix Ueberheide","orcid":"0000-0003-2512-0204","position":7,"is_corresponding":false},{"id":324094,"name":"Luke H. Chao","orcid":"0000-0002-4849-4148","position":8,"is_corresponding":false},{"id":324092,"name":"Sivakumar Boopathy","orcid":"0000-0003-0524-3338","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-19T01:19:10.334330Z","pmid":"38234813","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}