{"doi":"10.1101/2023.12.22.572991","title":"Dual carbon sequestration with photosynthetic living materials","abstract":"Abstract Natural ecosystems offer efficient pathways for carbon sequestration, serving as a resilient approach to remove CO 2 from the atmosphere with minimal environmental impact. However, the control of living systems outside of their native environments is often challenging. Here, we engineered a photosynthetic living material for dual CO 2 sequestration by immobilizing photosynthetic microorganisms within a printable polymeric network. The carbon concentrating mechanism of the cyanobacteria enabled accumulation of CO 2 within the cell, resulting in biomass production. Additionally, the metabolic production of OH - ions in the surrounding medium created an environment for the formation of insoluble carbonates via microbially-induced calcium carbonate precipitation (MICP). Digital design and fabrication of the living material ensured sufficient access to light and nutrient transport of the encapsulated cyanobacteria, which were essential for long-term viability (more than one year) as well as efficient photosynthesis and carbon sequestration. The photosynthetic living materials sequestered approximately 2.5 mg of CO 2 per gram of hydrogel material over 30 days via dual carbon sequestration, with 2.2 ± 0.9 mg stored as insoluble carbonates. Over an extended incubation period of 400 days, the living materials sequestered 26 ± 7 mg of CO 2 per gram of hydrogel material in the form of stable minerals. These findings highlight the potential of photosynthetic living materials for scalable carbon sequestration, carbon-neutral infrastructure, and green building materials. The simplicity of maintenance, coupled with its scalability nature, suggests broad applications of photosynthetic living materials as a complementary strategy to mitigate CO 2 emissions.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":395546,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9515,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1170834,"name":"Yifan Cui","orcid":"0000-0002-3772-4876","position":1,"is_corresponding":false},{"id":1170835,"name":"Andrea Shin Ling","orcid":"0000-0003-2383-4473","position":2,"is_corresponding":false},{"id":1170836,"name":"Felix Donat","orcid":"0000-0002-3940-9183","position":3,"is_corresponding":false},{"id":1170837,"name":"Stéphane Bernhard","orcid":"0000-0002-6534-6905","position":4,"is_corresponding":false},{"id":1170838,"name":"Margherita Bernero","orcid":"0009-0000-2327-3544","position":5,"is_corresponding":false},{"id":1171176,"name":"Akhil Areeckal","orcid":null,"position":6,"is_corresponding":false},{"id":1170839,"name":"Xiao‐Hua Qin","orcid":"0000-0001-8355-3230","position":7,"is_corresponding":false},{"id":491637,"name":"John Oakey","orcid":"0000-0003-1023-1964","position":8,"is_corresponding":false},{"id":1170840,"name":"Benjamin Dillenburger","orcid":"0000-0002-5153-2985","position":9,"is_corresponding":false},{"id":1170841,"name":"André R. Studart","orcid":"0000-0003-4205-8545","position":10,"is_corresponding":false},{"id":688039,"name":"Mark W. Tibbitt","orcid":"0000-0002-4917-7187","position":11,"is_corresponding":false},{"id":1170833,"name":"Dalia Dranseike","orcid":"0000-0002-5935-3666","position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":null,"created_at":"2026-07-19T01:19:22.857556Z","pmid":"38187760","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}