{"doi":"10.1101/2023.12.15.571945","title":"An RXLR effector targets ER-Golgi interface to induce ER stress and necrotic cell death","abstract":"Abstract To achieve successful colonization, the pathogen secretes hundreds of effectors into host cells to manipulate the host’s immune response. Despite numerous studies, the molecular mechanisms underlying effector-induced necrotic cell death remain elusive. In this study, we identified a novel virulent RXLR effector named Pc12 from P. capsici. Pc12 induces necrosis by triggering a distinct ER stress response through its interaction with Rab13-2. Unlike conventional hypersensitive response cell death associated with effector-triggered immunity, Pc12-induced cell death does not coincide with defense gene expression. Instead, it induces the aggregation of ER-resident proteins and confines secretory proteins within the ER. Pc12 interacts with Rab13-2, exhibiting a specific affinity for the active form of Rab13-2. Thus, the complex of Pc12 and Rab13-2 mimics the conformation of the inactive state of Rab13-2, subsequently recruiting the Rab-escort protein (REP). This process results in disruptions in vesicle formation within the ER-Golgi trafficking pathway. Furthermore, the substitution of a single amino acid of Rab13-2 structurally predicted to be crucial for the Pc12 interaction decreased the interaction with Pc12 while maintaining the interaction with REP1. These findings offer valuable insights into the ER stress-mediated cell death as well as a potential strategy for enhancing resistance against pathogens.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":401952,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9492,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1179332,"name":"Jesse Kaleku","orcid":"0000-0002-7089-1609","position":1,"is_corresponding":false},{"id":1179333,"name":"Jongchan Woo","orcid":"0000-0002-2994-6444","position":2,"is_corresponding":false},{"id":1179334,"name":"Hongshi Jin","orcid":"0000-0003-3283-1012","position":3,"is_corresponding":false},{"id":1179335,"name":"Hui Jeong Kang","orcid":"0000-0002-1347-8965","position":4,"is_corresponding":false},{"id":1179336,"name":"Minji Kang","orcid":"0000-0001-8118-3184","position":5,"is_corresponding":false},{"id":1179337,"name":"Haeun Kim","orcid":"0000-0002-6171-0650","position":6,"is_corresponding":false},{"id":1179338,"name":"Seungmee Jung","orcid":"0000-0003-2660-7663","position":7,"is_corresponding":false},{"id":74953,"name":"Cécile Segonzac","orcid":"0000-0002-5537-7556","position":8,"is_corresponding":false},{"id":1179339,"name":"Eunsook Park","orcid":"0000-0003-2984-3039","position":9,"is_corresponding":false},{"id":1179340,"name":"Doil Choi","orcid":"0000-0002-4366-3627","position":10,"is_corresponding":false},{"id":886710,"name":"Jihyun Kim","orcid":"0000-0003-0195-4319","position":0,"is_corresponding":true}],"reference_count":58,"raw_metadata":null,"created_at":"2026-07-19T01:20:23.920156Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}