{"doi":"10.1101/2023.12.15.571704","title":"An Integrated Optogenetic and Bioelectronic Platform for Regulating Cardiomyocyte Function","abstract":"Abstract We report an integrated optogenetic and bioelectronic platform for stable and long-term modulation and monitoring of cardiomyocyte function in vitro. Optogenetic inputs were achieved through expression of a photoactivatable adenylyl cyclase (bPAC), that when activated by blue light caused a dose-dependent and time-limited increase in autonomous cardiomyocyte beat rate. Bioelectronic readouts were achieved through an integrated planar multi-electrode array (MEA) that provided real-time readouts of electrophysiological activity from 32 spatially-distinct locations. Irradiation at 27 μW/mm 2 resulted in a ca. 14% increase in beat rate within 20-25 minutes, which remained stable for at least 2 hours. The beating rate could be cycled through repeated “on” and “off” states, and its magnitude was a monotonic function of irradiation intensity. Our integrated platform opens new avenues in bioelectronic medicine, including closedloop feedback systems, with potential applications for cardiac regulation including arrhythmia diagnosis and intervention. Teaser A system that integrates optogenetic stimulation and bioelectronic recording capabilities allows for on-demand regulation of cardiac cell function.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":415025,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9508,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":439681,"name":"Zijing Chen","orcid":"0000-0002-7996-2004","position":1,"is_corresponding":false},{"id":1197203,"name":"Yan-Ru Lai","orcid":null,"position":2,"is_corresponding":false},{"id":1197204,"name":"Megan Cote","orcid":null,"position":3,"is_corresponding":false},{"id":1196883,"name":"Akshita A. Rao","orcid":"0000-0001-7457-8188","position":4,"is_corresponding":false},{"id":572852,"name":"Haitao Liu","orcid":"0000-0003-1824-0304","position":5,"is_corresponding":false},{"id":385811,"name":"Emmanuel S. Tzanakakis","orcid":"0000-0002-6677-352X","position":6,"is_corresponding":false},{"id":377138,"name":"Brian P. Timko","orcid":"0000-0002-7958-8980","position":7,"is_corresponding":false},{"id":1196882,"name":"Olurotimi A. Bolonduro","orcid":"0000-0002-3210-0650","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-19T01:22:08.814145Z","pmid":"38168441","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}