{"doi":"10.1101/2023.11.12.566704","title":"Conserved 5-methyluridine tRNA modification modulates ribosome translocation","abstract":"ABSTRACT While the centrality of post-transcriptional modifications to RNA biology has long been acknowledged, the function of the vast majority of modified sites remains to be discovered. Illustrative of this, there is not yet a discrete biological role assigned for one the most highly conserved modifications, 5-methyluridine at position 54 in tRNAs (m 5 U54). Here, we uncover contributions of m 5 U54 to both tRNA maturation and protein synthesis. Our mass spectrometry analyses demonstrate that cells lacking the enzyme that installs m 5 U in the T-loop (TrmA in E. coli , Trm2 in S. cerevisiae ) exhibit altered tRNA modifications patterns. Furthermore, m 5 U54 deficient tRNAs are desensitized to small molecules that prevent translocation in vitro. This finding is consistent with our observations that, relative to wild-type cells, trm2 Δ cell growth and transcriptome-wide gene expression are less perturbed by translocation inhibitors. Together our data suggest a model in which m 5 U54 acts as an important modulator of tRNA maturation and translocation of the ribosome during protein synthesis.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":392732,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9489,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":863031,"name":"Monika K. Franco","orcid":"0000-0001-8059-8476","position":1,"is_corresponding":false},{"id":863030,"name":"Mehmet Tardu","orcid":"0000-0003-1674-5958","position":2,"is_corresponding":false},{"id":763466,"name":"T.J. Smith","orcid":"0000-0001-8444-6808","position":3,"is_corresponding":false},{"id":899015,"name":"Laura R. Snyder","orcid":null,"position":4,"is_corresponding":false},{"id":863029,"name":"Daniel E. Eyler","orcid":"0000-0002-6425-9770","position":5,"is_corresponding":false},{"id":351892,"name":"Yury S. Polikanov","orcid":"0000-0002-5064-0327","position":6,"is_corresponding":false},{"id":307439,"name":"Robert T. Kennedy","orcid":"0000-0003-2447-7471","position":7,"is_corresponding":false},{"id":828549,"name":"Rachel O. Niederer","orcid":"0000-0002-4574-0518","position":8,"is_corresponding":false},{"id":304098,"name":"Kristin S. Koutmou","orcid":"0000-0002-7763-9262","position":9,"is_corresponding":false},{"id":304096,"name":"Joshua D. Jones","orcid":"0000-0002-8811-1340","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":null,"created_at":"2026-07-19T01:18:56.730121Z","pmid":"37986750","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}