{"doi":"10.1101/2023.11.06.565822","title":"E-proteins set the threshold for optimal TCF1 expression during αβ T cell development","abstract":"Abstract Expression of T Cell Factor-1 (TCF1), encoded by Tcf7, regulates lineage fate decisions during T cell development. Here we demonstrate that E-proteins control the threshold of TCF1 expression required for development of T cells. E-proteins bind to five elements (EPEs) in the Tcf7 locus. The third element, EPE3, interacts directly with Tcf7 promoter in Hi-ChIP analyses, suggesting it is an active enhancer. CRISPR-ablation of EPE3 reduces TCF1 protein expression in precursor thymocytes by 2-fold and dramatically impairs development of αβ and γδ T cells. Single cell gene expression analysis identified differentiation blocks at multiple CD4 - CD8 - stages and subsequent transition to CD4 + CD8 + stage. These data identify E-proteins and EPE3 as critical for the optimal TCF1 expression required for T cell development.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":413688,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9579,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1194937,"name":"Bridget Aylward","orcid":"0000-0002-0377-2028","position":1,"is_corresponding":false},{"id":1194938,"name":"Fei Ma","orcid":"0000-0001-9432-1902","position":2,"is_corresponding":false},{"id":813230,"name":"Cheryl A. Sherman‐Baust","orcid":"0000-0001-7977-4698","position":3,"is_corresponding":false},{"id":299366,"name":"Laura B. Chopp","orcid":"0000-0001-6494-4439","position":4,"is_corresponding":false},{"id":61555,"name":"Susan A. Shinton","orcid":"0000-0003-2649-1904","position":5,"is_corresponding":false},{"id":519821,"name":"Roshni Roy","orcid":null,"position":6,"is_corresponding":false},{"id":689386,"name":"Shawn P. Fahl","orcid":"0000-0002-0489-2048","position":7,"is_corresponding":false},{"id":441296,"name":"Alejandra Contreras","orcid":"0000-0003-3653-5958","position":8,"is_corresponding":false},{"id":1194939,"name":"Byron Koenitzer","orcid":"0009-0004-1861-8711","position":9,"is_corresponding":false},{"id":262758,"name":"Parirokh Awasthi","orcid":"0000-0002-5831-9781","position":10,"is_corresponding":false},{"id":298507,"name":"Krystyna Mazan-Mamczarz","orcid":"0009-0005-1545-0500","position":11,"is_corresponding":false},{"id":275722,"name":"Supriyo De","orcid":"0000-0002-2075-7655","position":12,"is_corresponding":false},{"id":109799,"name":"Noah Ollikainen","orcid":"0000-0002-1174-2400","position":13,"is_corresponding":false},{"id":397071,"name":"Xiang Qiu","orcid":"0000-0001-9161-9033","position":14,"is_corresponding":false},{"id":299371,"name":"Rémy Bosselut","orcid":"0000-0003-2033-1790","position":15,"is_corresponding":false},{"id":264587,"name":"Ranjan Sen","orcid":"0000-0002-1916-3905","position":16,"is_corresponding":false},{"id":441297,"name":"David L. Wiest","orcid":"0000-0002-0792-3188","position":17,"is_corresponding":false},{"id":264588,"name":"Jyoti Misra Sen","orcid":"0000-0002-6365-0866","position":18,"is_corresponding":false},{"id":264586,"name":"Anjali Verma","orcid":"0000-0003-4031-5966","position":0,"is_corresponding":true}],"reference_count":50,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:21:57.641539Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}