{"doi":"10.1101/2023.11.05.565734","title":"Dengue virus exploits the host tRNA epitranscriptome to promote viral replication","abstract":"Abstract The 40-50 RNA modifications of the epitranscriptome regulate posttranscriptional gene expression. Here we show that flaviviruses hijack the host tRNA epitranscriptome to promote expression of pro-viral proteins, with tRNA-modifying ALKBH1 acting as a host restriction factor in dengue virus infection. Early in the infection of human Huh-7 cells, ALKBH1 and its tRNA products 5-formylcytidine (f 5 C) and 2’- O -methyl-5-formylcytidine (f 5 Cm) were reduced. ALKBH1 knockdown mimicked viral infection, but caused increased viral NS3 protein levels during infection, while ALKBH1 overexpression reduced NS3 levels and viral replication, and increased f 5 C and f 5 Cm. Viral NS5, but not host FTSJ1, increased f 5 Cm levels late in infection. Consistent with reports of impaired decoding of leucine UUA codon by f 5 Cm-modified tRNA Leu(CAA) , ALKBH1 knockdown induced translation of UUA-deficient transcripts, most having pro-viral functions. Our findings support a dynamic ALKBH1/f 5 Cm axis during dengue infection, with virally-induced remodeling of the proteome by tRNA reprogramming and codon-biased translation.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":390620,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9614,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1162899,"name":"Newman Siu Kwan Sze","orcid":null,"position":1,"is_corresponding":false},{"id":1162900,"name":"Yuka Suzuki","orcid":null,"position":2,"is_corresponding":false},{"id":1162418,"name":"Takayuki Ohira","orcid":"0000-0002-1487-1312","position":3,"is_corresponding":false},{"id":236036,"name":"Tsutomu Suzuki","orcid":"0000-0002-9731-1731","position":4,"is_corresponding":false},{"id":394551,"name":"Thomas J. Begley","orcid":"0000-0002-5641-7644","position":5,"is_corresponding":false},{"id":289582,"name":"Peter C. Dedon","orcid":"0000-0003-0011-3067","position":6,"is_corresponding":false},{"id":1162417,"name":"Cheryl Chan","orcid":"0000-0003-3388-0158","position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:18:37.743462Z","pmid":"37986976","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}