{"doi":"10.1101/2023.10.18.562080","title":"Long-Term <i>In Vivo</i> Molecular Monitoring Using Aptamer-Graphene Microtransistors","abstract":"Abstract Long-term, real-time molecular monitoring in complex biological environments is critical for our ability to understand, prevent, diagnose, and manage human diseases. Aptamer-based electrochemical biosensors possess the promise due to their generalizability and a high degree of selectivity. Nevertheless, the operation of existing aptamer-based biosensors in vivo is limited to a few hours. Here, we report a first-generation long-term in vivo molecular monitoring platform, named aptamer-graphene microtransistors (AGMs). The AGM incorporates a layer of pyrene- (polyethylene glycol)5-alcohol and DNase inhibitor-doped polyacrylamide hydrogel coating to reduce biofouling and aptamer degradation. As a demonstration of function and generalizability, the AGM achieves the detection of biomolecules such as dopamine and serotonin in undiluted whole blood at 37 °C for 11 days. Furthermore, the AGM successfully captures optically evoked dopamine release in vivo in mice for over one week and demonstrates the capability to monitor behaviorally-induced endogenous dopamine release even after eight days of implantation in freely moving mice. The results reported in this work establish the potential for chronic aptamer-based molecular monitoring platforms, and thus serve as a new benchmark for molecular monitoring using aptamer-based technology.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":390835,"datarank":0.3596842909197557,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.0,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9528,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":641105,"name":"Eric T. Zhang","orcid":"0000-0003-2614-905X","position":1,"is_corresponding":false},{"id":862011,"name":"Yingqi Qiang","orcid":"0000-0001-8763-2323","position":2,"is_corresponding":false},{"id":907479,"name":"Colin Esmonde","orcid":null,"position":3,"is_corresponding":false},{"id":282450,"name":"Xingchi Chen","orcid":"0009-0007-6296-5122","position":4,"is_corresponding":false},{"id":1152207,"name":"Zichao Wei","orcid":"0000-0001-5818-5077","position":5,"is_corresponding":false},{"id":862008,"name":"Yang Song","orcid":"0000-0003-0626-0850","position":6,"is_corresponding":false},{"id":1060272,"name":"Xincheng Zhang","orcid":"0000-0002-8182-7178","position":7,"is_corresponding":false},{"id":862010,"name":"Michael J. Schneider","orcid":"0000-0003-1435-1584","position":8,"is_corresponding":false},{"id":848174,"name":"Huijie Li","orcid":"0000-0002-8304-5241","position":9,"is_corresponding":false},{"id":862012,"name":"He Sun","orcid":"0000-0003-3701-9453","position":10,"is_corresponding":false},{"id":862600,"name":"Zhengyan Weng","orcid":null,"position":11,"is_corresponding":false},{"id":567333,"name":"Sabato Santaniello","orcid":"0000-0002-2133-9471","position":12,"is_corresponding":false},{"id":1162652,"name":"Jie He","orcid":"0009-0005-0291-9648","position":13,"is_corresponding":false},{"id":862014,"name":"Rebecca Y. Lai","orcid":"0000-0002-1732-9481","position":14,"is_corresponding":false},{"id":299433,"name":"Yan Li","orcid":"0000-0002-5938-8519","position":15,"is_corresponding":false},{"id":303878,"name":"Michael R. Bruchas","orcid":"0000-0003-4713-7816","position":16,"is_corresponding":false},{"id":787744,"name":"Yi Zhang","orcid":"0000-0002-0907-663X","position":17,"is_corresponding":false},{"id":862007,"name":"Guangfu Wu","orcid":"0000-0002-4714-4568","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:18:37.743462Z","pmid":"37905115","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}