{"doi":"10.1101/2023.10.06.561273","title":"Bacterial cell surface nanoenvironment requires a specialized chaperone to activate a peptidoglycan biosynthetic enzyme","abstract":"spores are produced inside the cytosol of a mother cell. Spore surface assembly requires the SpoVK protein in the mother cell, but its function is unknown. Here, we report that SpoVK is a dedicated chaperone from a distinct higher-order clade of AAA+ ATPases that activates the peptidoglycan glycosyltransferase MurG during sporulation, even though MurG does not normally require activation by a chaperone during vegetative growth. MurG redeploys to the spore surface during sporulation, where we show that the local pH is reduced and propose that this change in cytosolic nanoenvironment necessitates a specific chaperone for proper MurG function. Further, we show that SpoVK participates in a developmental checkpoint in which improper spore surface assembly inactivates SpoVK, which leads to sporulation arrest. The AAA+ ATPase clade containing SpoVK includes other dedicated chaperones involved in secretion, cell-envelope biosynthesis, and carbohydrate metabolism, suggesting that such fine-tuning might be a widespread feature of different subcellular nanoenvironments.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":412430,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9557,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1190358,"name":"Sylvia Chareyre","orcid":"0009-0001-5482-3817","position":1,"is_corresponding":false},{"id":561850,"name":"Vivek Anantharaman","orcid":"0000-0001-8395-0009","position":2,"is_corresponding":false},{"id":904939,"name":"Michael C. Gilmore","orcid":"0000-0002-6848-5134","position":3,"is_corresponding":false},{"id":575566,"name":"David L. Popham","orcid":"0000-0002-2614-143X","position":4,"is_corresponding":false},{"id":316673,"name":"Felipe Cava","orcid":"0000-0001-5995-718X","position":5,"is_corresponding":false},{"id":286376,"name":"L. Aravind","orcid":"0000-0003-0771-253X","position":6,"is_corresponding":false},{"id":386064,"name":"Kumaran S. Ramamurthi","orcid":"0000-0002-2335-3568","position":7,"is_corresponding":false},{"id":799857,"name":"Thomas Delerue","orcid":"0000-0002-8900-247X","position":0,"is_corresponding":true}],"reference_count":71,"raw_metadata":null,"created_at":"2026-07-19T01:21:50.261851Z","pmid":"37986874","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}