{"doi":"10.1101/2023.09.22.559026","title":"Target-based discovery of a broad spectrum flukicide","abstract":"Diseases caused by parasitic flatworms impart a considerable healthcare burden worldwide. Many of these diseases – for example, the parasitic blood fluke infection, schistosomiasis – are treated with the drug praziquantel (PZQ). However, PZQ is ineffective against disease caused by liver flukes from the genus Fasciola . This is due to a single amino acid change within the target of PZQ, a transient receptor potential ion channel (TRPM PZQ ), in Fasciola species. Here we identify benzamidoquinazolinone analogs that are active against Fasciola TRPM PZQ . Structure-activity studies define an optimized ligand (BZQ) that caused protracted paralysis and damage to the protective tegument of these liver flukes. BZQ also retained activity against Schistosoma mansoni comparable to PZQ and was active against TRPM PZQ orthologs in all profiled species of parasitic fluke. This broad spectrum activity was manifest as BZQ adopts a pose within the binding pocket of TRPM PZQ dependent on a ubiquitously conserved residue. BZQ therefore acts as a universal activator of trematode TRPM PZQ and a first-in-class, broad spectrum flukicide.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":391989,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9499,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":709866,"name":"Sang-Kyu Park","orcid":"0000-0002-3875-1227","position":1,"is_corresponding":false},{"id":1165224,"name":"Svenja Gramberg","orcid":"0009-0009-2108-4905","position":2,"is_corresponding":false},{"id":863164,"name":"Lisa Bauer","orcid":"0000-0002-9461-7284","position":3,"is_corresponding":false},{"id":636074,"name":"Claudia M. Rohr","orcid":"0000-0003-0065-7160","position":4,"is_corresponding":false},{"id":709864,"name":"Evgeny G. Chulkov","orcid":"0000-0001-8030-6031","position":5,"is_corresponding":false},{"id":249871,"name":"Emery Smith","orcid":"0000-0002-9217-6892","position":6,"is_corresponding":false},{"id":249872,"name":"Louis Scampavia","orcid":"0000-0002-7734-0105","position":7,"is_corresponding":false},{"id":249876,"name":"Timothy Spicer","orcid":"0000-0002-9080-4226","position":8,"is_corresponding":false},{"id":1007667,"name":"Simone Haeberlein","orcid":"0000-0002-1932-9696","position":9,"is_corresponding":false},{"id":606949,"name":"Jonathan S. Marchant","orcid":"0000-0001-6592-0877","position":10,"is_corresponding":false},{"id":430139,"name":"Daniel J. Sprague","orcid":"0000-0002-0709-3284","position":0,"is_corresponding":true}],"reference_count":26,"raw_metadata":null,"created_at":"2026-07-19T01:18:52.232318Z","pmid":"37790347","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}