{"doi":"10.1101/2023.09.19.558482","title":"Consistent specificity and efficiency of tamoxifen-mediated cre induction across ages","abstract":"Summary Temporally controlling cre recombination through tamoxifen (Tam) induction has many advantages for biomedical research. Most studies report Tam induction at early post-natal/juvenile (<2 m.o.) mouse ages, but age-related neurodegeneration and aging studies can require cre induction in older mice (>12 m.o.). While anecdotally reported as problematic, there are no published comparisons of Tam mediated cre induction at early and late ages. Here, microglial-specific Cx3cr1 creERT 2 mice were crossed to a floxed NuTRAP reporter to compare cre induction at early (3-6 m.o.) and late (20 m.o.) ages. Specificity and efficiency of microglial labeling at 21-22 m.o. were identical in mice induced with Tam at 3-6 m.o. or 20 m.o. of age. Age-related microglial translatomic changes were also similar regardless of Tam induction age. Each cre and flox mouse line should be validated independently, however, these findings demonstrate that Tam-mediated cre induction can be performed even into older mouse ages.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":411691,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9555,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":809567,"name":"Kevin D. Pham","orcid":null,"position":1,"is_corresponding":false},{"id":1024006,"name":"Sung-Hwan Ko","orcid":"0009-0000-8572-702X","position":2,"is_corresponding":false},{"id":1024005,"name":"Jillian E. J. Cox","orcid":"0009-0002-7213-094X","position":3,"is_corresponding":false},{"id":365198,"name":"Adeline H. Machalinski","orcid":null,"position":4,"is_corresponding":false},{"id":333910,"name":"Michael B. Stout","orcid":"0000-0002-9996-9123","position":5,"is_corresponding":false},{"id":333909,"name":"Amanda L. Sharpe","orcid":"0000-0003-3971-5946","position":6,"is_corresponding":false},{"id":333911,"name":"Michael J. Beckstead","orcid":"0000-0002-1574-530X","position":7,"is_corresponding":false},{"id":333902,"name":"Ana J. Chucair‐Elliott","orcid":"0000-0002-0172-1876","position":8,"is_corresponding":false},{"id":333903,"name":"Sarah R. Ocañas","orcid":"0000-0003-4281-0303","position":9,"is_corresponding":false},{"id":333912,"name":"Willard M. Freeman","orcid":"0000-0001-7027-999X","position":10,"is_corresponding":false},{"id":1024771,"name":"Collyn M. Kellogg","orcid":null,"position":0,"is_corresponding":true}],"reference_count":72,"raw_metadata":null,"created_at":"2026-07-19T01:21:42.971455Z","pmid":"37781585","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}