{"doi":"10.1101/2023.09.18.558286","title":"Plant-based production of diverse human milk oligosaccharides","abstract":"Human milk oligosaccharides (HMOs) are a diverse class of carbohydrates that aid in the health and development of infants. The vast health benefits of HMOs have made them a commercial target for microbial production; however, producing the ∼130 structurally diverse HMOs at scale has proven difficult. Here, we produce a vast diversity of HMOs by leveraging the robust carbohydrate anabolism of plants. This diversity includes high value HMOs, such as lacto-N-fucopentaose I, that have not yet been commercially produced using state-of-the-art microbial fermentative processes. HMOs produced in transgenic plants provided strong bifidogenic properties, indicating their ability to serve as a prebiotic supplement. Technoeconomic analyses demonstrate that producing HMOs in plants provides a path to the large-scale production of specific HMOs at lower prices than microbial production platforms. Our work demonstrates the promise in leveraging plants for the cheap and sustainable production of HMOs.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":397308,"datarank":0.2243656071444845,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.05957376384426805,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.05957376384426805,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":2,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9555,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1172904,"name":"Bruna Paviani","orcid":"0000-0001-6409-6994","position":1,"is_corresponding":false},{"id":629696,"name":"Garret Couture","orcid":"0000-0002-8947-4354","position":2,"is_corresponding":false},{"id":858097,"name":"Chad Masarweh","orcid":"0000-0003-2482-8972","position":3,"is_corresponding":false},{"id":895175,"name":"Ye Chen","orcid":"0000-0002-6379-1968","position":4,"is_corresponding":false},{"id":1172905,"name":"Yu‐Ping Huang","orcid":"0000-0001-8570-5963","position":5,"is_corresponding":false},{"id":235512,"name":"David A. Mills","orcid":"0000-0003-1913-9865","position":6,"is_corresponding":false},{"id":235510,"name":"Carlito B. Lebrilla","orcid":"0000-0001-7190-5323","position":7,"is_corresponding":false},{"id":235509,"name":"Daniela Barile","orcid":"0000-0002-3889-1596","position":8,"is_corresponding":false},{"id":1172906,"name":"Minliang Yang","orcid":"0000-0002-4670-7947","position":9,"is_corresponding":false},{"id":416296,"name":"Patrick M. Shih","orcid":"0000-0002-2119-3345","position":10,"is_corresponding":false},{"id":670547,"name":"Collin R. Barnum","orcid":"0000-0003-0978-7105","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:19:35.497854Z","pmid":"37786679","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}