{"doi":"10.1101/2023.08.29.555369","title":"Lymphatic vessel transit seeds precursors to cytotoxic resident memory T cells in skin draining lymph nodes","abstract":"Abstract Resident memory T cells (T RM ) provide rapid, localized protection in peripheral tissues to pathogens and cancer. While T RM are also found in lymph nodes (LN), how they develop during primary infection and their functional significance remains largely unknown. Here, we track the anatomical distribution of anti-viral CD8 + T cells as they simultaneously seed skin and LN T RM using a model of skin infection with restricted antigen distribution. We find exquisite localization of LN T RM to the draining LN of infected skin. LN T RM formation depends on lymphatic transport and specifically egress of effector CD8 + T cells that appear poised for residence as early as 12 days post infection. Effector CD8 + T cell transit through skin is necessary and sufficient to populate LN T RM in draining LNs, a process reinforced by antigen encounter in skin. Importantly, we demonstrate that LN T RM are sufficient to provide protection against pathogenic rechallenge. These data support a model whereby a subset of tissue infiltrating CD8 + T cells egress during viral clearance, and establish regional protection in the draining lymphatic basin as a mechanism to prevent pathogen spread. One Sentence Summary T cell egress out of virally infected skin via afferent lymphatic vessels seeds CD8 + resident memory T cells in the draining lymph node.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":391957,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9506,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1161699,"name":"Austin C. Schultz","orcid":"0009-0000-2406-8497","position":1,"is_corresponding":false},{"id":1018641,"name":"Ines Delclaux","orcid":"0009-0001-6420-2934","position":2,"is_corresponding":false},{"id":1165181,"name":"Vanessa Cristaldi","orcid":"0000-0001-7345-3383","position":3,"is_corresponding":false},{"id":465805,"name":"Madeline J. Churchill","orcid":"0000-0001-7624-4894","position":4,"is_corresponding":false},{"id":516590,"name":"Amanda W. Lund","orcid":"0000-0001-7389-9983","position":5,"is_corresponding":false},{"id":829481,"name":"Taylor A. Heim","orcid":"0000-0003-4128-5754","position":0,"is_corresponding":true}],"reference_count":53,"raw_metadata":null,"created_at":"2026-07-19T01:18:52.232318Z","pmid":"37693469","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}