{"doi":"10.1101/2023.08.28.555221","title":"Metagenomic analysis of individual mosquitos reveals the ecology of insect viruses","abstract":"Mosquito transmitted viruses are responsible for an increasing burden of human disease. Despite this, little is known about the diversity and ecology of viruses within individual mosquito hosts. Using a meta-transcriptomic approach, we analysed the virome of 2,438 individual mosquitos (79 species), spanning ~4000 km along latitudes and longitudes in China. From these data we identified 393 core viral species associated with mosquitos, including seven (putative) arbovirus species. We identified potential species and geographic hotspots of viral richness and arbovirus occurrence, and demonstrated that host phylogeny had a strong impact on the composition of individual mosquito viromes. Our data revealed a large number of viruses shared among mosquito species or genera, expanding our knowledge of host specificity of insect-associated viruses. We also detected multiple virus species that were widespread throughout the country, possibly facilitated by long-distance mosquito migrations. Together, our results greatly expand the known mosquito virome, linked the viral diversity at the scale of individual insects to that at a country-wide scale, and offered unique insights into the ecology of viruses of insect vectors.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":390806,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8759,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1162612,"name":"Hailong Zhao","orcid":"0000-0002-5008-3225","position":1,"is_corresponding":false},{"id":1019668,"name":"Qinyu Gou","orcid":"0000-0003-1311-2901","position":2,"is_corresponding":false},{"id":1163042,"name":"Peibo Shi","orcid":null,"position":3,"is_corresponding":false},{"id":39964,"name":"Jun-Hua Tian","orcid":null,"position":4,"is_corresponding":false},{"id":681235,"name":"Yun Feng","orcid":null,"position":5,"is_corresponding":false},{"id":848123,"name":"Kun Li","orcid":"0000-0002-2026-9969","position":6,"is_corresponding":false},{"id":681237,"name":"Weihong Yang","orcid":null,"position":7,"is_corresponding":false},{"id":1162613,"name":"De Wu","orcid":"0000-0003-1873-9914","position":8,"is_corresponding":false},{"id":1163043,"name":"Guangpeng Tang","orcid":null,"position":9,"is_corresponding":false},{"id":687001,"name":"Bing Zhang","orcid":"0000-0002-1750-1659","position":10,"is_corresponding":false},{"id":1163044,"name":"Zirui Ren","orcid":null,"position":11,"is_corresponding":false},{"id":1163045,"name":"Shiqin Peng","orcid":null,"position":12,"is_corresponding":false},{"id":1163046,"name":"Gengyan Luo","orcid":null,"position":13,"is_corresponding":false},{"id":1163047,"name":"Shi-Jia Le","orcid":null,"position":14,"is_corresponding":false},{"id":1019669,"name":"Gen-Yang Xin","orcid":"0000-0002-4401-8531","position":15,"is_corresponding":false},{"id":289608,"name":"Jing Wang","orcid":"0000-0002-7120-0857","position":16,"is_corresponding":false},{"id":1162614,"name":"Xin Hou","orcid":"0000-0002-1816-1887","position":17,"is_corresponding":false},{"id":1163048,"name":"Minwu Peng","orcid":null,"position":18,"is_corresponding":false},{"id":1163049,"name":"Jian-bin Kong","orcid":null,"position":19,"is_corresponding":false},{"id":1162615,"name":"Xinxin Chen","orcid":"0000-0003-3929-2314","position":20,"is_corresponding":false},{"id":1162616,"name":"Chunhui Yang","orcid":"0000-0002-3012-4501","position":21,"is_corresponding":false},{"id":1163050,"name":"Shi-Qiang Mei","orcid":null,"position":22,"is_corresponding":false},{"id":1163051,"name":"Yu-qi Liao","orcid":null,"position":23,"is_corresponding":false},{"id":765303,"name":"Jing-Xia Cheng","orcid":null,"position":24,"is_corresponding":false},{"id":1162617,"name":"Juan Wang","orcid":"0000-0002-4656-6865","position":25,"is_corresponding":false},{"id":1163052,"name":"Chaolemen","orcid":null,"position":26,"is_corresponding":false},{"id":925734,"name":"Yuhui Wu","orcid":"0000-0002-2147-3857","position":27,"is_corresponding":false},{"id":1162618,"name":"Jianbo Wang","orcid":"0000-0002-0092-0937","position":28,"is_corresponding":false},{"id":1162619,"name":"Tongqing An","orcid":"0000-0003-4619-4587","position":29,"is_corresponding":false},{"id":1162620,"name":"Xinyi Huang","orcid":"0000-0002-2827-1860","position":30,"is_corresponding":false},{"id":810265,"name":"John‐Sebastian Eden","orcid":"0000-0003-1374-3551","position":31,"is_corresponding":false},{"id":13730,"name":"Jun Li","orcid":"0000-0001-7218-429X","position":32,"is_corresponding":false},{"id":234883,"name":"Jun Li","orcid":"0000-0002-3257-9315","position":33,"is_corresponding":false},{"id":860590,"name":"Deyin Guo","orcid":"0000-0002-8297-0814","position":34,"is_corresponding":false},{"id":680500,"name":"Guodong Liang","orcid":"0000-0003-1278-8731","position":35,"is_corresponding":false},{"id":270852,"name":"Xin Jin","orcid":"0000-0001-7554-4975","position":36,"is_corresponding":false},{"id":39971,"name":"Edward C. 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