{"doi":"10.1101/2023.08.09.552630","title":"Blood-based Nano-QuIC: Inhibitor-resistant Detection of Seeding Activity in Patients Diagnosed with Parkinson Disease","abstract":"A hallmark of α-synucleinopathies (e.g. Parkinson disease) is the misfolding and aggregation of α-synuclein in tissues and biological fluids. Protein amplification assays like real-time quaking-induced conversion (RT-QuIC) are sensitive yet currently limited to semi-invasive sample types such as cerebrospinal fluid because more accessible samples, such as blood, contain inhibitors. Here, we show that Nanoparticle-enhanced Quaking-induced Conversion (Nano-QuIC) can double the speed of reactions spiked with misfolded α-synuclein while increasing sensitivity 100-fold in human plasma. Nano-QuIC detected spike concentrations down to 90 pg/ml in lysed whole blood, while reactions without nanoparticles (RT-QuIC) failed to have any detection due to the presence of strong inhibitors. Moreover, Nano-QuIC showed increased seeding activity in plasma samples from Parkinson disease patients (n=4) versus healthy controls (n=4). This sets the groundwork for the noninvasive diagnostic use of Nano-QuIC, potentially enabling early disease detection and management through blood-based testing.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":397039,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9596,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1172672,"name":"Hyeonjeong Jeong","orcid":"0000-0002-5094-5390","position":1,"is_corresponding":false},{"id":292314,"name":"Manci Li","orcid":"0000-0003-3930-7117","position":2,"is_corresponding":false},{"id":1173039,"name":"Hyerim Ahn","orcid":null,"position":3,"is_corresponding":false},{"id":269265,"name":"Ann M. Schmeichel","orcid":null,"position":4,"is_corresponding":false},{"id":635983,"name":"Pinaki Misra","orcid":"0000-0001-5185-1890","position":5,"is_corresponding":false},{"id":1172673,"name":"Danni Li","orcid":"0009-0007-5589-6405","position":6,"is_corresponding":false},{"id":259158,"name":"Rodolfo Savica","orcid":"0000-0002-2543-0627","position":7,"is_corresponding":false},{"id":267188,"name":"Phillip A. Low","orcid":"0000-0002-5386-4764","position":8,"is_corresponding":false},{"id":267185,"name":"Wolfgang Singer","orcid":"0000-0003-1263-6850","position":9,"is_corresponding":false},{"id":564410,"name":"Peter A. Larsen","orcid":"0000-0002-3634-3625","position":10,"is_corresponding":false},{"id":1172674,"name":"Hye Yoon Park","orcid":"0000-0003-4704-8178","position":11,"is_corresponding":false},{"id":526684,"name":"Sang‐Hyun Oh","orcid":"0000-0002-6992-5007","position":12,"is_corresponding":false},{"id":1172671,"name":"Peter R. Christenson","orcid":"0000-0003-3979-7945","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:19:35.497854Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}