{"doi":"10.1101/2023.07.25.550454","title":"Massively Concurrent Sub-Cellular Traction Force Videography enabled by Single-Pixel Optical Tracers (SPOTs)","abstract":"Abstract We report a large field-of-view and high-speed videography platform for measuring the sub-cellular traction forces of more than 10,000 biological cells over 13mm 2 at 83 frames per second. Our Single-Pixel Optical Tracers (SPOT) tool uses 2-dimensional diffraction gratings embedded into a soft substrate to convert cells’ mechanical traction stress into optical colors detectable by a video camera. The platform measures the sub-cellular traction forces of diverse cell types, including tightly connected tissue sheets and near isolated cells. We used this platform to explore the mechanical wave propagation in a tightly connected sheet of Neonatal Rat Ventricular Myocytes (NRVMs) and discovered that the activation time of some tissue regions are heterogeneous from the overall spiral wave behavior of the cardiac wave. One-Sentence Summary An optical platform for fast, concurrent measurements of cell mechanics at 83 frames per second, over a large area of 13mm 2 .","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":409805,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.955,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":291456,"name":"Yijie Wang","orcid":"0000-0002-2681-0138","position":1,"is_corresponding":false},{"id":783914,"name":"Xiongfeng Zhu","orcid":"0000-0003-3517-9170","position":2,"is_corresponding":false},{"id":1190016,"name":"Felipe Nanni Mendes","orcid":null,"position":3,"is_corresponding":false},{"id":494678,"name":"Pei‐Shan Chung","orcid":null,"position":4,"is_corresponding":false},{"id":1189688,"name":"Yu Ting Chow","orcid":"0000-0001-9851-6459","position":5,"is_corresponding":false},{"id":511607,"name":"Tianxing Man","orcid":"0000-0001-5079-3844","position":6,"is_corresponding":false},{"id":1189689,"name":"Hsin Lan","orcid":"0009-0007-8715-7340","position":7,"is_corresponding":false},{"id":682040,"name":"Yen-Ju Lin","orcid":"0000-0002-8848-7778","position":8,"is_corresponding":false},{"id":339036,"name":"Xiang Zhang","orcid":"0000-0002-3272-894X","position":9,"is_corresponding":false},{"id":1189690,"name":"Xiaohe Zhang","orcid":"0000-0002-0124-4475","position":10,"is_corresponding":false},{"id":244618,"name":"Thang L. Nguyen","orcid":"0000-0002-9809-3836","position":11,"is_corresponding":false},{"id":319928,"name":"Reza Ardehali","orcid":"0000-0003-1318-4016","position":12,"is_corresponding":false},{"id":244626,"name":"Michael A. Teitell","orcid":"0000-0002-4495-8750","position":13,"is_corresponding":false},{"id":244631,"name":"Arjun Deb","orcid":"0000-0003-0978-2611","position":14,"is_corresponding":false},{"id":244627,"name":"Pei‐Yu Chiou","orcid":"0000-0003-4677-2447","position":15,"is_corresponding":false},{"id":244619,"name":"Xing Haw Marvin Tan","orcid":"0000-0002-5674-2906","position":0,"is_corresponding":true}],"reference_count":31,"raw_metadata":null,"created_at":"2026-07-19T01:21:31.144858Z","pmid":"37546726","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}