{"doi":"10.1101/2023.07.25.550441","title":"Origin of eukaryotic-like Vps23 shapes an ancient functional interplay between ESCRT and ubiquitin system in Asgard archaea","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>Functional interplay between the endosomal sorting complexes required for transport (ESCRT) and ubiquitin system underlies the ubiquitin-dependent sorting pathway, a specific trait of eukaryotic endomembrane system. Yet, its evolutionary origin remains unclear. Here, we show that a novel UEV-Vps23 family protein, that contains UEV and Vps23 domains, mediates an ancient ESCRT and ubiquitin system interplay in Asgard archaea. The UEV binds ubiquitin with high-affinity, making the UEV-Vps23 a sensor for sorting ubiquitinated cargo. A steadiness box in the Vps23 domain undergoes ubiquitination through a eukaryotic-like Asgard E1, E2, and RING E3 cascade. The UEV-Vps23 can switch between autoinhibited and active forms, by which likely regulates the ESCRT and ubiquitin system interplay. Furthermore, the shared sequence and structural homology among the UEV-Vps23, eukaryotic Vps23 and archaeal CdvA, implying that these proteins share a common evolutionary origin. Together, this work presents evidence that the ESCRT and ubiquitin system interplay had arisen early in Asgard evolution, antedating emergence of endomembrane system in eukaryogenesis.</jats:p>\n                <jats:sec>\n                  <jats:title>Teaser</jats:title>\n                  <jats:p>The ESCRT and ubiquitin system interplay, a specific trait of eukaryotic endomembrane system, likely inherited from an Asgard archaeal ancestor.</jats:p>\n                </jats:sec>","journal":null,"year":null,"id":669933,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1376861,"name":"Siyu Zhang","orcid":"0009-0005-5051-993X","position":1,"is_corresponding":false},{"id":1213708,"name":"Yang Liu","orcid":"0000-0001-6235-200X","position":2,"is_corresponding":false},{"id":1241421,"name":"Runyue Xia","orcid":"0000-0003-4017-4788","position":3,"is_corresponding":false},{"id":560690,"name":"Meng Li","orcid":"0000-0001-8675-0758","position":4,"is_corresponding":false},{"id":1176124,"name":"Zhongyi Lu","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Origin of eukaryotic-like Vps23 shapes an ancient functional interplay between ESCRT and ubiquitin system in Asgard archaea","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>Functional interplay between the endosomal sorting complexes required for transport (ESCRT) and ubiquitin system underlies the ubiquitin-dependent sorting pathway, a specific trait of eukaryotic endomembrane system. Yet, its evolutionary origin remains unclear. Here, we show that a novel UEV-Vps23 family protein, that contains UEV and Vps23 domains, mediates an ancient ESCRT and ubiquitin system interplay in Asgard archaea. The UEV binds ubiquitin with high-affinity, making the UEV-Vps23 a sensor for sorting ubiquitinated cargo. A steadiness box in the Vps23 domain undergoes ubiquitination through a eukaryotic-like Asgard E1, E2, and RING E3 cascade. The UEV-Vps23 can switch between autoinhibited and active forms, by which likely regulates the ESCRT and ubiquitin system interplay. Furthermore, the shared sequence and structural homology among the UEV-Vps23, eukaryotic Vps23 and archaeal CdvA, implying that these proteins share a common evolutionary origin. Together, this work presents evidence that the ESCRT and ubiquitin system interplay had arisen early in Asgard evolution, antedating emergence of endomembrane system in eukaryogenesis.</jats:p>\n                <jats:sec>\n                  <jats:title>Teaser</jats:title>\n                  <jats:p>The ESCRT and ubiquitin system interplay, a specific trait of eukaryotic endomembrane system, likely inherited from an Asgard archaeal ancestor.</jats:p>\n                </jats:sec>","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19162232","pmcid":null,"openalex_id":"https://openalex.org/W4385276346","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2024,"count":1}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2023/07/26/2023.07.25.550441.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2023/07/26/2023.07.25.550441.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2023.07.25.550441","host_type":"publisher"},{"url":"https://doi.org/10.1101/2023.07.25.550441","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","Genetic and Kidney Cyst Diseases","Protist diversity and phylogeny"],"mesh_terms":[],"keywords":["ESCRT","Endomembrane system","Ubiquitin","Biology","Vacuolar protein sorting","Cell biology","Computational biology","Genetics","Endosome","Gene","Endoplasmic reticulum"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-15T09:26:36.179582Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}