{"doi":"10.1101/2023.07.19.549812","title":"The complexity and dynamics of <i>in organello</i> translation assessed by high-resolution mitochondrial ribosome profiling","abstract":"Abstract Since mitochondrial translation serves the essential subunits of the OXPHOS complex that produces ATP, exhaustive, quantitative, and high-resolution delineation of mitoribosome traversal is needed. Here, we developed a technique for high-resolution mitochondrial ribosome profiling and revealed the intricate regulation of mammals in organello translation. Our approach assessed the stoichiometry and kinetics of mitochondrial translation flux, such as the number of mitoribosomes on a transcript and the elongation rate, initiation rate, and lifetime rounds of translation of individual transcripts. We also surveyed the impacts of modifications at the anticodon stem loop in mt-tRNAs, including all possible modifications at the 34th position, by deleting the corresponding enzymes and harnessing patient-derived cells. Moreover, a retapamulin-assisted derivative and mito-disome profiling revealed cryptic translation initiation sites at subcognate codons and programmed mitoribosome collision sites across the mitochondrial transcriptome. Our work provides a useful platform for investigating protein synthesis within the energy powerhouse of the cell.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2023,"id":390079,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9481,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":236031,"name":"Mari Mito","orcid":"0000-0003-2564-6273","position":1,"is_corresponding":false},{"id":1161365,"name":"Haruna Yamashiro","orcid":"0009-0000-0783-3222","position":2,"is_corresponding":false},{"id":1161366,"name":"Kotaro Tomuro","orcid":"0009-0008-3513-4507","position":3,"is_corresponding":false},{"id":1161367,"name":"Haruna Tani","orcid":"0009-0006-1277-8145","position":4,"is_corresponding":false},{"id":650333,"name":"Kazuhito Tomizawa","orcid":"0000-0002-5663-2627","position":5,"is_corresponding":false},{"id":650327,"name":"Takeshi Chujo","orcid":"0000-0003-2393-5776","position":6,"is_corresponding":false},{"id":1066675,"name":"Asuteka Nagao","orcid":"0000-0002-9152-1527","position":7,"is_corresponding":false},{"id":1161368,"name":"Takeo Suzuki","orcid":"0000-0002-2747-4076","position":8,"is_corresponding":false},{"id":650334,"name":"Fan‐Yan Wei","orcid":"0000-0002-4843-724X","position":9,"is_corresponding":false},{"id":245404,"name":"Yuichi Shichino","orcid":"0000-0002-0093-1185","position":10,"is_corresponding":false},{"id":236036,"name":"Tsutomu Suzuki","orcid":"0000-0002-9731-1731","position":11,"is_corresponding":false},{"id":17833,"name":"Shintaro Iwasaki","orcid":"0000-0001-7724-3754","position":12,"is_corresponding":false},{"id":1161364,"name":"Taisei Wakigawa","orcid":"0000-0003-4369-3364","position":0,"is_corresponding":true}],"reference_count":139,"raw_metadata":null,"created_at":"2026-07-19T01:18:32.854511Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}