{"doi":"10.1101/2023.06.24.546416","title":"Fluorogenesis: Inducing Fluorescence in a Non-Fluorescent Protein Through Photoinduced Chromophore Transfer of a Genetically Encoded Chromophore","abstract":"<jats:title>ABSTRACT</jats:title>\n                <jats:p>\n                  Fluorescent proteins, while essential for bioimaging, are limited to visualizing cellular localization without offering additional functionality. We report for the first time a strategy to expand the chemical, structural, and functional diversity of fluorescent proteins by harnessing light to induce red fluorescence in a previously non-fluorescent protein. We accomplish this by inducing the transfer of the genetically encoded chromophore from a photocleavable protein (PhoCl1) to a non-fluorescent kinase (\n                  <jats:italic>Mj</jats:italic>\n                  RibK) inducing red fluorescence in the latter. We have employed analytical and spectroscopic techniques to validate the presence of red fluorescence in\n                  <jats:italic>Mj</jats:italic>\n                  RibK. Furthermore, molecular dynamics simulations were carried out to investigate the amino acid residues of\n                  <jats:italic>Mj</jats:italic>\n                  RibK involved in the generation of red fluorescence. Finally, we demonstrate the ability of the red fluorescent\n                  <jats:italic>Mj</jats:italic>\n                  RibK to operate as a cyclable high-temperature sensor. We anticipate that this light-induced chromophore transfer strategy will open new possibilities for developing multifunctional genetically encoded fluorescent sensors.\n                </jats:p>","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":null,"id":25742,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":150673,"name":"Reman Kumar Singh","orcid":"0000-0001-6160-4586","position":1,"is_corresponding":false},{"id":150674,"name":"Manisha Ojha","orcid":"0009-0002-7741-9339","position":2,"is_corresponding":false},{"id":150675,"name":"Karthik Pushpavanam","orcid":"0000-0001-6312-2572","position":3,"is_corresponding":false},{"id":150672,"name":"Yashwant Kumar","orcid":"0000-0002-6822-4241","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24523987","pmcid":null,"openalex_id":"https://openalex.org/W4382117342","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.biorxiv.org/content/biorxiv/early/2023/06/25/2023.06.24.546416.full.pdf","host_type":"repository"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2023/06/25/2023.06.24.546416.full.pdf","host_type":"GREEN"},{"url":"https://www.biorxiv.org/content/biorxiv/early/2023/06/25/2023.06.24.546416.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.1101/2023.06.24.546416","host_type":"publisher"},{"url":"http://dx.doi.org/10.1101/2023.06.24.546416","host_type":"repository"}],"fields_of_study":["Advanced Fluorescence Microscopy Techniques","bioluminescence and chemiluminescence research","Photoreceptor and optogenetics research","Biology","Chemistry"],"mesh_terms":[],"keywords":["Fluorescence","Chromophore","Green fluorescent protein","Fluorescent protein","Biophysics","Chemistry","Bimolecular fluorescence complementation","Protein tag","Fluorescence in the life sciences","Förster resonance energy transfer","Photochemistry","Biochemistry","Biology","Recombinant DNA","Fusion protein","Gene","Optics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Industry, innovation and infrastructure"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-08T07:36:51.594864Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}